Experimental Lung Transplantation Related With HIF-1, VEGF, ROS. Assessment of HIF-1alpha, VEGF, and Reactive Oxygen Species After Competitive Blockade of Chetomin for Lung Transplantation in Rats.

IF 1.9 4区医学 Q3 PHYSIOLOGY

Physiological research Pub Date : 2024-11-19

C Bravo-Reyna, A Zentella, J Ventura-Gallegos, G Torres-Villalobos, V Miranda-Galván, J Alanis-Mendizabal, J Escobar-Valderrama, C Nava, N Díaz-Martínez, T Bliskunova, V Morales-De Los Santos

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引用次数: 0

Abstract

Primary graft failure occurs 15 to 30 % of the time after transplantation. Although there have been improvements in preserving the lungs in good condition, there have not been studies on the regulation of transcription factors.

Methods: We carried out an experimental study involving lung transplantation to indirectly evaluate reactive oxygen species (ROS) production and VEGF expression by competitive blockade of HIF-1alpha with chetomin. There were 5 groups: Group-1: Lung blocks were perfused with 0.9 % SSF, immediately harvested, and preserved. Group-2 (I-T): Immediate transplantation and then reperfusion for 1 h. Group-3 (I-R): Lung blocks were harvested and preserved in LPD solution for 6 h and reperfused for 1 h. Group-4 (DMSO): Lung blocks were treated for 4 h with DMSO, preserved for 6 h and transplanted to a receptor treated with DMSO. Group-5 (chetomin): Lung blocks were treated for 4 h with chetomin, preserved for 6 h and transplanted to a receptor treated with chetomin. ROS, mRNA, and protein levels of HIF-1alpha and EG-VEGF were determined.

Results: The DMSO and chetomin groups had significantly lower ROS levels. Compared with those in the I-R group, the chetomin group exhibited the lowest level of HIF-1alpha.

Conclusions: Addition of chetomin to the donor and the receptor results in a significant reduction in HIF-1A, VEGF and ROS.

本刊更多论文

实验性肺移植与 HIF-1、血管内皮生长因子和活性氧有关。竞争性阻断切托明用于大鼠肺移植后对 HIF-1α、血管内皮生长因子和活性氧的评估

原发性移植失败发生率为移植后的15%至30%。虽然在保持肺部良好状态方面有所改进，但还没有关于转录因子调控的研究：我们进行了一项涉及肺移植的实验研究，通过 Chetomin 竞争性阻断 HIF-1alpha 来间接评估活性氧（ROS）的产生和血管内皮生长因子（VEGF）的表达。共分为 5 组：第 1 组：用 0.9 % SSF 灌注肺块，立即收获并保存。第二组（I-T）：第 3 组（I-R）：收获肺块并在 LPD 溶液中保存 6 小时，然后再灌注 1 小时。第四组（DMSO）：用二甲基亚砜处理肺块 4 小时，保存 6 小时，然后移植到用二甲基亚砜处理的受体上。第 5 组（chetomin）：用 chetomin 处理肺块 4 小时，保存 6 小时，然后移植到用 chetomin 处理的受体上。测定 ROS、HIF-1alpha 和 EG-VEGF 的 mRNA 和蛋白水平：结果：DMSO组和chetomin组的ROS水平明显较低。与 I-R 组相比，chetomin 组的 HIF-1alpha 水平最低：结论：在供体和受体中添加 chetomin 可显著降低 HIF-1A、血管内皮生长因子和 ROS。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physiological research 医学-生理学

CiteScore

4.00

自引率

4.80%

发文量

108

审稿时长

3 months

期刊介绍： Physiological Research is a peer reviewed Open Access journal that publishes articles on normal and pathological physiology, biochemistry, biophysics, and pharmacology. Authors can submit original, previously unpublished research articles, review articles, rapid or short communications. Instructions for Authors - Respect the instructions carefully when submitting your manuscript. Submitted manuscripts or revised manuscripts that do not follow these Instructions will not be included into the peer-review process. The articles are available in full versions as pdf files beginning with volume 40, 1991. The journal publishes the online Ahead of Print /Pre-Press version of the articles that are searchable in Medline and can be cited.