Characterization and Comparative Genomic Analysis of vB_BceM_CEP1: A Novel Temperate Bacteriophage Infecting Burkholderia cepacia Complex.

IF 3.3 4区 生物学 Q2 MICROBIOLOGY
Journal of Microbiology Pub Date : 2024-11-01 Epub Date: 2024-11-18 DOI:10.1007/s12275-024-00185-2
Momen Askoura, Eslam K Fahmy, Safya E Esmaeel, Wael A H Hegazy, Aliaa Abdelghafar
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引用次数: 0

Abstract

The increasing prevalence of multidrug-resistant bacteria imminently threatens public health and jeopardizes nearly all aspects of modern medicine. The Burkholderia cepacia complex (Bcc) comprises Burkholderia cepacia and the related species of Gram-negative bacteria. Members of the Bcc group are opportunistic pathogens responsible for various chronic illnesses, including cystic fibrosis and chronic granulomatous disease. Phage therapy is emerging as a potential solution to combat the antimicrobial resistance crisis. In this study, a temperate phage vB_BceM_CEP1 was isolated from sewage and fully characterized. Transmission electron microscopy indicated that vB_BceM_CEP1 belongs to the family Peduoviridae. The isolated phage demonstrated enhanced environmental stability and antibiofilm potential. One-step growth analysis revealed a latent period of 30 min and an average burst size of 139 plaque-forming units per cell. The genome of vB_BceM_CEP1 consists of 32,486 bp with a GC content of 62.05%. A total of 40 open reading frames were annotated in the phage genome, and none of the predicted genes was annotated as tRNA. Notably, genes associated with antibiotic resistance, host virulence factors, and toxins were absent from the vB_BceM_CEP1 genome. Based on its unique phenotype and phylogeny, the isolated phage vB_BceM_CEP1 is classified as a new temperate phage with lytic activity. The findings of this study enhance our understanding of the diversity of Bcc phages.

vB_BceM_CEP1:一种感染伯克霍尔德氏菌复合体的新型温带噬菌体的特征和基因组比较分析。
耐多药细菌的日益流行迫在眉睫地威胁着公众健康,并危及现代医学的几乎所有方面。伯克霍尔德氏菌复合菌(Bcc)包括伯克霍尔德氏菌和相关的革兰氏阴性菌种。伯克霍尔德头孢菌素复合菌(Bcc)是由伯克霍尔德头孢菌素杆菌(Burkholderia cepacia)和相关的革兰氏阴性菌组成。噬菌体疗法正在成为应对抗菌药耐药性危机的潜在解决方案。本研究从污水中分离出一种温带噬菌体 vB_BceM_CEP1,并对其进行了全面鉴定。透射电子显微镜显示 vB_BceM_CEP1 属于 Peduoviridae 科。分离出的噬菌体具有更强的环境稳定性和抗生物膜潜力。一步生长分析表明,其潜伏期为 30 分钟,每个细胞的平均迸发大小为 139 个斑块形成单位。vB_BceM_CEP1 的基因组由 32,486 bp 组成,GC 含量为 62.05%。噬菌体基因组中共注释了 40 个开放阅读框,没有一个预测基因被注释为 tRNA。值得注意的是,vB_BceM_CEP1 基因组中没有与抗生素抗性、宿主毒力因子和毒素相关的基因。根据其独特的表型和系统发育,分离出的 vB_BceM_CEP1 噬菌体被归类为具有溶菌活性的新型温带噬菌体。这项研究的发现加深了我们对 Bcc 噬菌体多样性的了解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Microbiology
Journal of Microbiology 生物-微生物学
CiteScore
5.70
自引率
3.30%
发文量
0
审稿时长
3 months
期刊介绍: Publishes papers that deal with research on microorganisms, including archaea, bacteria, yeasts, fungi, microalgae, protozoa, and simple eukaryotic microorganisms. Topics considered for publication include Microbial Systematics, Evolutionary Microbiology, Microbial Ecology, Environmental Microbiology, Microbial Genetics, Genomics, Molecular Biology, Microbial Physiology, Biochemistry, Microbial Pathogenesis, Host-Microbe Interaction, Systems Microbiology, Synthetic Microbiology, Bioinformatics and Virology. Manuscripts dealing with simple identification of microorganism(s), cloning of a known gene and its expression in a microbial host, and clinical statistics will not be considered for publication by JM.
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