Glucose-1-phosphate thymidylyltransferase promotes the production of 3-O-α-mycarosylerythronolide B in Streptomyces coelicolor.

IF 3.2 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of Applied Microbiology Pub Date : 2024-11-18 DOI:10.1093/jambio/lxae291

Hong Gao, Swen Langer, Tony Larson, Matthew A Gregory, Margaret C M Smith

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引用次数: 0

Abstract

Aims: The main objective of this study was to produce erythronolide B (EB) and 3-O-α-mycarosylerythronolide B (MEB) in Streptomyces coelicolor and enhance the MEB production by expressing the glucose-1-phosphate thymidylyltransferase (RfbA).

Methods and results: We expressed eryF and eryB genes (eryBII, eryBIII, eryBIV, eryBV, eryBVI and eryBVII) to produce EB and MEB. The expression was confirmed by quantitative real-time PCR. Furthermore, the MEB's production was improved by more than 100-fold by expressing an enzyme, RfbA, which is absent from the erythromycin gene cluster, to promote the biosynthesis of TDP-L-mycarose. We discuss the feasibility of alternative Streptomyces species for erythromycin production based on the presence or absence of RfbA.

Conclusions: The RbfA enzyme from Saccharopolyspora erythraea was expressed in S. coelicolor M1152 along with the MEB biosynthesis pathway, resulting in a large increase in MEB production (>100-fold).

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葡萄糖-1-磷酸胸苷基转移酶促进共生链霉菌产生 3-O-α-mycarosylerythronolide B。

目的：本研究的主要目的是在壳斗链霉（Streptomyces coelicolor）中生产红曲内酯B（EB）和3-O-α-mycarosylerythronolide B（MEB），并通过表达葡萄糖-1-磷酸胸苷基转移酶（RfbA）来提高MEB的产量：我们表达了eryF和eryB基因（eryBII、eryBIII、eryBIV、eryBV、eryBVI和eryBVII）以产生EB和MEB。实时定量 PCR 对其表达进行了确认。此外，通过表达红霉素基因簇中缺少的一种酶 RfbA 来促进 TDP-L-mycarose 的生物合成，MEB 的产量提高了 100 倍以上。我们根据 RfbA 的存在与否，讨论了生产红霉素的其他链霉菌种的可行性：结论：在S.coelicolor M1152中表达了来自Saccharopolyspora erythraea的RbfA酶以及MEB生物合成途径，结果MEB产量大幅增加（>100倍）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Microbiology 生物-生物工程与应用微生物

CiteScore

7.30

自引率

2.50%

发文量

427

审稿时长

2.7 months

期刊介绍： Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.