Diversity and networking of uni-cyanobacterial cultures and associated heterotrophic bacteria from the benthic microbial mat of a desert hydrothermal spring.

IF 3.5 3区 生物学 Q2 MICROBIOLOGY
Khaoula Lassoued, Mouna Mahjoubi, Elias Asimakis, Naima Bel Mokhtar, Panagiota Stathopoulou, Refka Ben Hamouda, Olfa Bousselmi, Ramona Marasco, Ahmed Slaheddine Masmoudi, Daniele Daffonchio, George Tsiamis, Ameur Cherif
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Abstract

Thermal springs harbour microorganisms, often dominated by cyanobacteria, which form biofilms and microbial mats. These phototrophic organisms release organic exudates into their immediate surroundings, attracting heterotrophic bacteria that contribute to the diversity and functioning of these ecosystems. In this study, the microbial mats from a hydrothermal pool in the Ksar Ghilane oasis in the Grand Erg Oriental of the Desert Tunisia were collected to obtain cyanobacterial cultures formed by single cyanobacterial species. High-throughput analysis showed that while the microbial mat hosted diverse cyanobacteria, laboratory cultures selectively enriched cyanobacteria from the Leptolyngbya, Nodosilinea, and Arthronema. Per each of these genera, multiple non-axenic uni-cyanobacterial cultures were established, totalling 41 cultures. Cyanobacteria taxa mediated the assembly of distinct heterotrophic bacterial communities, with members of the Proteobacteria and Actinobacteria phyla dominating. The bacterial communities of uni-cyanobacterial cultures were densely interconnected, with heterotrophic bacteria preferentially co-occurring with each other. Our study highlighted the complex structures of non-axenic uni-cyanobacterial cultures, where taxonomically distinct cyanobacteria consistently associate with specific groups of heterotrophic bacteria. The observed associations were likely driven by common selection pressures in the laboratory, such as cultivation conditions and specific hosts, and may not necessarily reflect the microbial dynamic occurring in the spring microbial mats.

沙漠热液泉底栖微生物垫中单青霉菌培养物和相关异养菌的多样性和网络化。
温泉蕴藏着微生物,通常以蓝藻为主,蓝藻会形成生物膜和微生物垫。这些光养生物会向周围环境释放有机渗出物,吸引异养细菌,从而促进这些生态系统的多样性和功能。在这项研究中,研究人员收集了突尼斯东方大沙漠中 Ksar Ghilane 绿洲热液池中的微生物垫,以获得由单一蓝藻物种形成的蓝藻培养物。高通量分析表明,虽然微生物垫上有多种蓝藻,但实验室培养物选择性地富集了 Leptolyngbya、Nodosilinea 和 Arthronema 的蓝藻。每个属都建立了多个非同轴单蓝藻培养物,共计 41 个培养物。蓝藻类群促成了独特的异养细菌群落的形成,其中以变形菌门和放线菌门的成员为主。单蓝藻培养物的细菌群落之间相互联系紧密,异养细菌优先相互共生。我们的研究突显了非同轴单蓝藻培养物的复杂结构,在这些培养物中,分类学上不同的蓝藻始终与特定的异养菌群结合在一起。观察到的关联可能是由实验室中的共同选择压力(如培养条件和特定宿主)驱动的,并不一定能反映春季微生物垫中的微生物动态。
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来源期刊
FEMS microbiology ecology
FEMS microbiology ecology 生物-微生物学
CiteScore
7.50
自引率
2.40%
发文量
132
审稿时长
3 months
期刊介绍: FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology. - Application of ecological theory to microbial ecology - Interactions and signalling between microorganisms and with plants and animals - Interactions between microorganisms and their physicochemical enviornment - Microbial aspects of biogeochemical cycles and processes - Microbial community ecology - Phylogenetic and functional diversity of microbial communities - Evolutionary biology of microorganisms
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