Potential PDE4A inhibition-mediated neuroprotective effects of psoralidin.

IF 3.3 4区医学 Q1 Medicine

European review for medical and pharmacological sciences Pub Date : 2024-11-01 DOI:10.26355/eurrev_202411_36913

E Uzunhisarcıklı

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引用次数: 0

Abstract

Objective: This study aimed to investigate the effect of psoralidin, a natural phenolic coumarin compound, on MK-801-induced neurotoxicity that may cause Alzheimer's disease and to determine the phosphodiesterase (PDE)-related molecular mechanism of action.

Materials and methods: In this study, neurotoxicity was performed using the MK-801 in the HT-22 cell line. The effects of compounds on the proliferation of HT-22 cells were determined by Real-Time Cell Analysis (RTCA). After measuring the total protein concentration, the PDE4A protein level was determined using the Western blot method.

Results: Psoralidin (100, 200, 400 µM) has been shown to have a neuroprotective effect against MK-801-induced neurotoxicity, as indicated by Real-Time Cell Analysis. In HT-22 cells, the half maximal effective concentration (EC50) value of psoralidin was calculated to be 230.4 µM, IC50 value of MK-801 was calculated to be 62.4 µM at 24 hours. It has been determined that psoralidin (200, 400 µM) inhibits PDE4A by using the Western blot method.

Conclusions: This research uncovers that psoralidin has neuroprotective effects in MK801-associated accumulation of the excitatory amino acid glutamate neurodegeneration and Alzheimer's disease.

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补骨脂素潜在的 PDE4A 抑制介导的神经保护作用

研究目的本研究旨在探讨天然酚类香豆素化合物补骨脂素对MK-801诱导的可能导致阿尔茨海默病的神经毒性的影响，并确定与磷酸二酯酶（PDE）相关的分子作用机制：本研究使用 MK-801 在 HT-22 细胞系中进行神经毒性实验。化合物对 HT-22 细胞增殖的影响通过实时细胞分析法（RTCA）进行测定。测定总蛋白浓度后，用 Western 印迹法测定 PDE4A 蛋白水平：实时细胞分析显示，补骨脂素（100、200、400 µM）对 MK-801 诱导的神经毒性有保护作用。在 HT-22 细胞中，计算出补骨脂素的半最大有效浓度（EC50）值为 230.4 µM，而 MK-801 在 24 小时内的 IC50 值为 62.4 µM。采用 Western 印迹法测定了补骨脂素（200、400 µM）对 PDE4A 的抑制作用：本研究发现，补骨脂素对与 MK801 相关的兴奋性氨基酸谷氨酸神经变性积累和阿尔茨海默病具有神经保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European review for medical and pharmacological sciences 医学-药学

CiteScore

5.30

自引率

6.10%

发文量

906

审稿时长

2-4 weeks

期刊介绍： European Review for Medical and Pharmacological Sciences, a fortnightly journal, acts as an information exchange tool on several aspects of medical and pharmacological sciences. It publishes reviews, original articles, and results from original research. The purposes of the Journal are to encourage interdisciplinary discussions and to contribute to the advancement of medicine. European Review for Medical and Pharmacological Sciences includes: -Editorials- Reviews- Original articles- Trials- Brief communications- Case reports (only if of particular interest and accompanied by a short review)