Copper Dysmetabolism is Connected to Epithelial-Mesenchymal Transition: A Pilot Study in Colorectal Cancer Patients.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Rosanna Squitti, Vincenzo Tondolo, Amit Pal, Gianluca Rizzo, Samanta Arijit, Hoque Mehboob, Laura di Veroli, Piera Catalano, Marco Della Ventura, Gioia Mastromoro, Luisa Rossi, Mauro Rongioletti, Anastasia De Luca
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Abstract

Colorectal cancer (CRC) is among the most diagnosed cancers worldwide, whose risk of mortality is associated with the development of metastases to the liver, lungs, and peritoneum. Of note, CRC is highly dependent on copper to sustain its proliferation and aggressiveness. Copper acts not only as a pivotal cofactor for several cuproproteins but also as an allosteric modulator of kinases essential to fulfill the epithelial-to-mesenchymal-transition (EMT), the main mechanism driving cancer cell spreading. System biology identified the APP and SOD1 genes among the top 10 genes shared between CRC and copper metabolism, as confirmed by the upregulation of the protein/mRNA levels of APP observed in CRC tissues. The significant increase of copper found in the sera of CRC patients was paralleled by a strong reduction of copper in the CRC tissues, in agreement with the decreased level of the high-affinity copper transporter CTR1 mRNA (SLC31A1) and LOXL2. As expected, in CRC tissues the mesenchymal marker fibronectin was significantly increased, whereas vimentin and vinculin protein levels were decreased compared to adjacent healthy mucosa. Interestingly, correlation analysis showed an interconnection between vinculin and both CCS and APP. A positive correlation was also observed between APP mRNA and both CDH1 and SOD1 mRNAs. Overall, we demonstrate a correlation between cell copper imbalance and CRC progression via EMT. The results obtained lay the scientific basis for further investigation to describe the kinetics of copper dysregulation during CRC progression and to identify the main cuproproteins involved in the modulation of EMT.

铜代谢紊乱与上皮-间质转化有关:结直肠癌患者的试点研究。
结肠直肠癌(CRC)是全球确诊率最高的癌症之一,其死亡风险与肝脏、肺部和腹膜的转移有关。值得注意的是,CRC 高度依赖铜来维持其增殖和侵袭性。铜不仅是几种铜蛋白的关键辅助因子,还是实现上皮细胞向间质细胞转变(EMT)所必需的激酶的异构调节剂,而EMT是驱动癌细胞扩散的主要机制。系统生物学研究发现,APP 和 SOD1 基因是 CRC 与铜代谢共有的前 10 个基因之一,CRC 组织中观察到的 APP 蛋白/RNA 水平上调也证实了这一点。在 CRC 患者血清中发现铜含量明显增加的同时,CRC 组织中的铜含量却大幅减少,这与高亲和性铜转运体 CTR1 mRNA(SLC31A1)和 LOXL2 水平的降低是一致的。不出所料,与邻近的健康粘膜相比,CRC 组织中的间质标志物纤连蛋白显著增加,而波形蛋白和长链蛋白水平则有所下降。有趣的是,相关分析表明 vinculin 与 CCS 和 APP 之间存在相互联系。APP mRNA 与 CDH1 和 SOD1 mRNA 之间也存在正相关。总之,我们证明了细胞铜失衡与 CRC 通过 EMT 进展之间的相关性。这些结果为进一步研究提供了科学依据,有助于描述 CRC 进展过程中铜失调的动力学,并确定参与 EMT 调节的主要铜蛋白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biological Trace Element Research
Biological Trace Element Research 生物-内分泌学与代谢
CiteScore
8.70
自引率
10.30%
发文量
459
审稿时长
2 months
期刊介绍: Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.
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