Genome wide identification of BjSWEET gene family and drought response analysis of BjSWEET12 and BjSWEET17 genes in Brassica juncea.

IF 4.3 2区生物学 Q1 PLANT SCIENCES

BMC Plant Biology Pub Date : 2024-11-19 DOI:10.1186/s12870-024-05815-w

Shuangping Heng, Jingjuan He, Xinyu Zhu, Jiayu Cai, Mengke Fu, Shaoheng Zhang, Wei Zeng, Feng Xing, Guangzhi Mao

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引用次数: 0

Abstract

Background: Sugars Will Eventually be Exported Transporter (SWEET) gene family is a unique type of sugar transporter that plays a vital role in metabolic regulation, growth, development, and stress response in multiple species. This study aimed to systematically identify the SWEET gene family members and detect the regulation of gene expression and their potential roles of the SWEET gene family in Brassica juncea.

Results: A total of 66 BjSWEET (Brassica juncea Sugar Will Eventually be Exported Transporter) genes distributed across 17 chromosomes were identified. The gene structure and motifs were relatively conserved, with all members containing the MtN3/saliva domain. Phylogenetic analysis revealed that the SWEET gene family can be classified into four subfamilies (Clades I, II, III, and IV). Collinearity analysis revealed that there were 118 pairs of segment duplicates, indicating that some BjSWEET genes were obtained via segmental duplication. The promoter regions of the BjSWEET genes contained many plant hormone-related response elements, stress-related response elements, growth and development elements, and light-responsive regulatory elements. Furthermore, analysis of the expression profiles revealed that the expression levels of the BjSWEET genes differed among the eight different tissues. qRT‒PCR analysis of six selected BjSWEET genes revealed that the expression levels of BjSWEET17.2, BjSWEET17.4, BjSWEET12.2, and BjSWEET12.3 were significantly upregulated under drought treatment, suggesting that these genes may respond to drought stress in B. juncea.

Conclusion: This study systematically identified and analyzed the SWEET gene family members in B. juncea for the first time, laying the foundation for further research on the molecular mechanisms of drought resistance in B. juncea and providing theoretical guidance for the application of these genes in other species.

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Brassica juncea 中 BjSWEET 基因家族的全基因组鉴定及 BjSWEET12 和 BjSWEET17 基因的干旱响应分析。

背景：糖类最终将被输出转运体（SWEET）基因家族是一种独特的糖类转运体，在多种物种的代谢调节、生长、发育和应激反应中发挥着重要作用。本研究旨在系统鉴定甘蓝中的 SWEET 基因家族成员，并检测 SWEET 基因家族在甘蓝中的基因表达调控及其潜在作用：结果：共鉴定出分布在 17 条染色体上的 66 个 BjSWEET（Brassica juncea Sugar Will eventually be Exported Transporter）基因。基因结构和结构域相对保守，所有成员都含有 MtN3/saliva结构域。系统发育分析表明，SWEET 基因家族可分为四个亚家族（支系 I、II、III 和 IV）。共线性分析显示，有 118 对片段重复，表明一些 BjSWEET 基因是通过片段重复获得的。BjSWEET 基因的启动子区域含有许多植物激素相关反应元件、胁迫相关反应元件、生长发育元件和光反应调控元件。对所选的六个 BjSWEET 基因进行 qRT-PCR 分析发现，在干旱处理下，BjSWEET17.2、BjSWEET17.4、BjSWEET12.2 和 BjSWEET12.3 的表达水平显著上调，这表明这些基因可能对杜仲的干旱胁迫做出了响应：本研究首次系统地鉴定和分析了君子兰中的 SWEET 基因家族成员，为进一步研究君子兰的抗旱分子机制奠定了基础，并为这些基因在其他物种中的应用提供了理论指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Plant Biology 生物-植物科学

CiteScore

8.40

自引率

3.80%

发文量

539

审稿时长

3.8 months

期刊介绍： BMC Plant Biology is an open access, peer-reviewed journal that considers articles on all aspects of plant biology, including molecular, cellular, tissue, organ and whole organism research.