Isolation, characterization, and activity of the polysaccharides in Bulbophyllum kwangtumgense Schltr.

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2024-11-16 DOI:10.1016/j.ijbiomac.2024.137382

Liuyue Xiao, Jiaojiao Hou, Ping Chen, Xiao Tan, Xiangxiang Qin, Jing Nie, Hua Zhu, Shian Zhong

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引用次数: 0

Abstract

A microwave-assisted method was used to extract the polysaccharide from Bulbophyllumkwangtumgense Schltr, and the optimum extraction conditions were determined by orthogonal experiments. The purified polysaccharide component BKP-1 had an obvious polysaccharide characteristic structure on the infrared spectrum and was mainly composed of xylose and glucuronic acid at a ratio of 0.568:0.432. Its molecular weight distribution (polydispersity index was 0.58) was uniform with 1.92× 10⁶ Da. The main structural skeleton of BKP-1 was →4)-β-D-Xylp-(1 → [4)-β-D-Xylp-(1]₄ → 4)-α-D-GlcAp-(1 → [4)-α-D-GlcAp-(1]₂→. BKP-1 could clear certain free radicals, among which the hydroxyl free radical scavenging ability was the best, with a clearance rate of 61.46 %. Furthermore, BKP-1 significantly modulated the secretion of both pro-inflammatory and anti-inflammatory cytokines at a concentration of 125 μg/mL. Notably, the mechanism BKP-1 exhibiting inhibitory effects were related to regulating of the NF-κB signaling pathway.

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Bulbophyllum kwangtumgense Schltr.

采用微波辅助法提取了Bulbophyllumkwangtumgense Schltr的多糖，并通过正交实验确定了最佳提取条件。纯化的多糖成分 BKP-1 在红外光谱上具有明显的多糖特征结构，主要由木糖和葡萄糖醛酸组成，二者的比例为 0.568:0.432。其分子量分布均匀（多分散指数为 0.58），为 1.92× 106 Da。BKP-1 的主要结构骨架为→4)-β-D-Xylp-(1→[4)-β-D-Xylp-(1]4→4)-α-D-GlcAp-(1→[4)-α-D-GlcAp-(1]2→。BKP-1 能清除某些自由基，其中清除羟自由基的能力最强，清除率为 61.46%。此外，在 125 μg/mL 的浓度下，BKP-1 还能明显调节促炎和抗炎细胞因子的分泌。值得注意的是，BKP-1 发挥抑制作用的机制与调节 NF-κB 信号通路有关。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.