The potential Impact of bacterial probiotics on ruminal greenhouse gases production in vitro of dietary Delonix regia seeds in rams and steers.

Abstract

This study aimed to evaluate the influence of probiotic bacteria (Pediococcus acidilactici BX-B122 and Bacillus coagulans BX-B118) on methane, carbon monoxide, and hydrogen sulfide, and fermentation profile of dietary Delonix regia seeds in ruminant. Ruminal contents from slaughtered rams and steers were used as inoculum for in vitro fermentation system. The total gas, methane, carbon monoxide, and hydrogen sulfide volume, as well as pH and dry matter degradability, were quantified in three fermentation cycles. Probiotic bacteria reduced the production of methane and hydrogen sulfide, while also increasing (P < 0.05) dry matter biodegradability, short-chain fatty acids, and metabolizable energy in both rams and steers. Delonix regia seeds at 6, 12, and 18% reduced total gas production. Higher production of methane and carbon monoxide was observed in rams compared to steers. Interestingly, no impact (P > 0.05) on the pH of the ruminal contents was found in Delonix regia seeds alone or in combination with probiotics. However, higher (P < 0.05) methane conversion efficiency (i.e., ratios of methane: short-chain fatty acids, methane: metabolizable energy, and methane: organic matter) was observed in experimental diets with Delonix regia seeds compared to diets containing both Delonix regia seeds and probiotic bacteria. In conclusion, dietary inclusion of 6, 12, and 18% of Delonix regia seeds with probiotic bacteria (Pediococcus acidilactici BX-B122 and Bacillus coagulans BX-B118) can mitigate the production of methane and hydrogen sulfide, while also increasing dry matter biodegradability, short-chain fatty acids, and metabolizable energy both ruminant animals.