Metabolism Interaction Between Bacillus cereus SESY and Brassica napus Contributes to Enhance Host Selenium Absorption and Accumulation.

Abstract

The use of beneficial bacteria to enhance selenium absorption in crops has been widely studied. However, it is unclear how the interaction between bacteria and plants affects selenium absorption in crops. Here, pot experiments and Murashige and Skoog medium (MS) experiments were performed. Transcriptomic analyses were used to reveal the interaction between Bacillus cereus SESY and Brassica napus. The results indicated that B. cereus SESY can significantly increase the biomass and selenium content of B. napus. The genes related to the colonization, IAA synthesis, and l-cysteine synthesis and metabolism of B. cereus SESY were significantly stimulated by B. napus through transcriptional regulation. Further verification results showed that l-cysteine increased selenium content in B. napus roots and shoots by 62.9% and 88.4%, respectively. B. cereus SESY and l-cysteine consistently regulated the relative expression level of genes involved in plant hormone, amino acid metabolism, selenium absorption, and Se enzymatic and nonenzymatic metabolic pathway of B. napus. These genes were significantly correlated with selenium content and biomass of B. napus (p < 0.05). Overall, IAA biosynthesis, and l-cysteine biosynthesis and metabolism in B. cereus SESY stimulated by interactions triggered molecular and metabolic responses of B. napus, underpinning host selenium absorption and accumulation.