Dual drug co-encapsulation of bevacizumab and pemetrexed clocked polymeric nanoparticles improves antiproliferative activity and apoptosis induction in liver cancer cells

IF 2.7 3区化学 Q2 POLYMER SCIENCE

Journal of Applied Polymer Science Pub Date : 2024-09-25 DOI:10.1002/app.56262

Subbulakshmi Ganesan, Vijay J. Upadhye, Damanjeet Aulakh, Sunita Bishnoi, Arunachalam Chinnathambi, Tahani Awad Alahmadi, Suha Mujahed Abudoleh, Palanisamy Arulselvan, Indumathi Thangavelu

{"title":"Dual drug co-encapsulation of bevacizumab and pemetrexed clocked polymeric nanoparticles improves antiproliferative activity and apoptosis induction in liver cancer cells","authors":"Subbulakshmi Ganesan, Vijay J. Upadhye, Damanjeet Aulakh, Sunita Bishnoi, Arunachalam Chinnathambi, Tahani Awad Alahmadi, Suha Mujahed Abudoleh, Palanisamy Arulselvan, Indumathi Thangavelu","doi":"10.1002/app.56262","DOIUrl":null,"url":null,"abstract":"<p>Nanoparticle (NP) enabled approaches have been employed for chemotherapeutic administration due to their capacity to regulate drug release and reduce side effects. Additionally, these methods can use several drugs concurrently and impede the proliferation of cancer cells that have developed resistance. Bevacizumab (BVZ) and pemetrexed (PEM) have demonstrated encouraging outcomes in the treatment and management of cancer. This work investigates the combined antiproliferative efficacy of BVZ and PEM co-loaded PLGA-PEG NPs (BVZ/PEM@PLGA-PEG NPs) against HepG2 liver cancerous cells. The BVZ/PEM@PLGA-PEG exhibited a sphere-shaped and consistent nanosized distribution. In addition, we evaluated the potential mechanisms for inhibiting cell growth and inducing apoptosis using DAPI staining and cell cycle study. The beneficial combined antiproliferative activity and the apoptosis pathway were detected in the HepG2 cells exposed to BVZ/PEM@PLGA-PEG NPs. Our study determined that the combinational drug treatment of BVZ/PEM@PLGA-PEG NPs has a significant effect on promoting the effectiveness of liver cancer treatment.</p>","PeriodicalId":183,"journal":{"name":"Journal of Applied Polymer Science","volume":"141 47","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Polymer Science","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/app.56262","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Nanoparticle (NP) enabled approaches have been employed for chemotherapeutic administration due to their capacity to regulate drug release and reduce side effects. Additionally, these methods can use several drugs concurrently and impede the proliferation of cancer cells that have developed resistance. Bevacizumab (BVZ) and pemetrexed (PEM) have demonstrated encouraging outcomes in the treatment and management of cancer. This work investigates the combined antiproliferative efficacy of BVZ and PEM co-loaded PLGA-PEG NPs (BVZ/PEM@PLGA-PEG NPs) against HepG2 liver cancerous cells. The BVZ/PEM@PLGA-PEG exhibited a sphere-shaped and consistent nanosized distribution. In addition, we evaluated the potential mechanisms for inhibiting cell growth and inducing apoptosis using DAPI staining and cell cycle study. The beneficial combined antiproliferative activity and the apoptosis pathway were detected in the HepG2 cells exposed to BVZ/PEM@PLGA-PEG NPs. Our study determined that the combinational drug treatment of BVZ/PEM@PLGA-PEG NPs has a significant effect on promoting the effectiveness of liver cancer treatment.

Abstract Image

查看原文本刊更多论文

贝伐珠单抗和培美曲塞双药共包合的时钟聚合物纳米颗粒提高了肝癌细胞的抗增殖活性和凋亡诱导能力

由于纳米粒子（NP）具有调节药物释放和减少副作用的能力，因此已被用于化疗给药。此外，这些方法还能同时使用多种药物，并抑制产生抗药性的癌细胞的增殖。贝伐单抗（BVZ）和培美曲塞（PEM）在癌症治疗和管理方面取得了令人鼓舞的成果。本研究探讨了 BVZ 和 PEM 共负载 PLGA-PEG NPs（BVZ/PEM@PLGA-PEG NPs）对 HepG2 肝癌细胞的联合抗增殖功效。BVZ/PEM@PLGA-PEG呈现出球形和一致的纳米级分布。此外，我们还利用 DAPI 染色和细胞周期研究评估了抑制细胞生长和诱导细胞凋亡的潜在机制。在暴露于 BVZ/PEM@PLGA-PEG NPs 的 HepG2 细胞中检测到了有益的联合抗增殖活性和凋亡途径。我们的研究确定，BVZ/PEM@PLGA-PEG NPs 的联合药物治疗对促进肝癌治疗效果具有显著作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Applied Polymer Science 化学-高分子科学

CiteScore

5.70

自引率

10.00%

发文量

1280

审稿时长

2.7 months

期刊介绍： The Journal of Applied Polymer Science is the largest peer-reviewed publication in polymers, #3 by total citations, and features results with real-world impact on membranes, polysaccharides, and much more.