Phytochemical composition and anti-oxidant, anti-Alzheimer and anti-inflammatory activities of pomegranate fruit as function of variety and ripening

IF 2.2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Herbal Medicine Pub Date : 2024-10-19 DOI:10.1016/j.hermed.2024.100960

Jalila Bekir, Mohamed Mars

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引用次数: 0

Abstract

Introduction

Medicinal plants are considered as rich resources of secondary metabolites and have been used for treating diseases. Pomegranate (Punica granatum) is considered ‘a pharmacy unto itself’. The current study aimed to evaluate the effect of ripening and variety factors on the antioxidant activity of pomegranate fruits and their capacity to inhibit three enzymes involved in degenerative diseases.

Methods

The fruits were collected from four Tunisian pomegranate varieties (Espagnoule, Rafrafi, Zaghwani and Zehri) during three maturity stages. Phytochemical content, the antioxidant activity by DPPH and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assays and acetylcholinesterase, butyrylcholinesterase and 15-lipoxygenase inhibition activities were carried out.

Results

The unripe fruits were found to be the richest in terms of total phenolic content. Total flavonoids, tannins, anthocyanins and acetylcholinesterase, butyrylcholinesterase and 15-lipoxygenase inhibition activities varied greatly during ripening process and within varieties. It was found that unripe fruits of Zehri (IC₅₀ = 2.2 µg/ml by 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) assay) and Rafrafi (IC₅₀ = 73.4 µg/ml by 2, 2-diphenyl-l-picrylhydrazyl assay) varieties exhibited the highest antioxidant capacities. The strongest extract against acetylcholinesterase and butyrylcholinesterase was found to be the unripe fruit extract of Espagnoule variety with IC₅₀ values of 86.7 and 58.0 µg/ml, respectively. The ethanolic extract from the unripe fruit of Zehri pomegranate variety inhibited moderately 15-lipoxygenase activity with an IC₅₀ value of 63.0 µg/ml.

Conclusion

The current study supported the popular use of pomegranate fruits, and highlighted also the potential effect of variety and ripening factors on their chemical composition and in vitro biological activities.

查看原文本刊更多论文

石榴果实的植物化学成分及抗氧化、抗老年痴呆和抗炎活性与品种和成熟度的关系

导言药用植物被认为是次生代谢物的丰富资源，一直被用于治疗疾病。石榴（Punica granatum）被认为是 "自成一体的药房"。本研究旨在评估成熟和品种因素对石榴果实抗氧化活性的影响，以及石榴果实抑制参与退化性疾病的三种酶的能力。采用 DPPH 和 2,2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）检测法检测植物化学成分含量、抗氧化活性以及乙酰胆碱酯酶、丁酰胆碱酯酶和 15-脂氧合酶抑制活性。总黄酮、单宁、花青素以及乙酰胆碱酯酶、丁酰胆碱酯酶和 15-脂氧合酶抑制活性在成熟过程中和不同品种之间存在很大差异。研究发现，Zehri（通过 2,2'-偶氮-双（3-乙基苯并噻唑啉-6-磺酸）测定法得出的 IC50 = 2.2 µg/ml ）和 Rafrafi（通过 2,2-二苯基-l-苦基肼测定法得出的 IC50 = 73.4 µg/ml）这两个品种的未成熟果实表现出最高的抗氧化能力。对乙酰胆碱酯酶和丁酰胆碱酯酶抗性最强的提取物是 Espagnoule 品种的未熟果实提取物，IC50 值分别为 86.7 和 58.0 µg/ml。从 Zehri 石榴品种的未成熟果实中提取的乙醇提取物对 15-脂氧合酶活性有中度抑制作用，IC50 值为 63.0 µg/ml。

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来源期刊

Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

3.90

自引率

0.00%

发文量

期刊介绍： The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.