Supercritical CO2 green solvent extraction of Nepeta crispa: Evaluation of process optimization, chemical analysis, and biological activity

IF 3.4 3区工程技术 Q2 CHEMISTRY, PHYSICAL

Journal of Supercritical Fluids Pub Date : 2024-11-04 DOI:10.1016/j.supflu.2024.106451

Saeid Matinfard , Hamid Tavakolipour , Gholamhossein Sodeifian , Seyed Ali Sajadian , Ramezan Kalvandi

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Abstract

This research investigates the extraction of compounds from Nepeta crispa (N. crispa), utilizing supercritical carbon dioxide green solvent, for the first time. Using RSM, the study examines the impact of pressure (15–25 MPa), temperature (313–333 K), and co-solvent (0.5–3.5%) on the extraction yield. The antioxidant activity of the essential oil is assessed through Ferric Reducing Antioxidant Power (FRAP) assay, while its antibacterial properties are evaluated against four bacterial strains. The SCFE method achieved a maximum yield of 1.812 % at optimum conditions (P= 25 MPa, T= 313 K and co-solvent= 3.5%). GC-Mass analysis revealed that the primary constituents of N. crispa essential oil which are 1,8-cineol and 4aα,7α,7aα-nepetalactone in both extraction methods. The antioxidant and antibacterial results of N. crispa showed the superiority of SC-CO₂ over hydrodistillation method. These observed antioxidant and antibacterial attributes highlight the plant’s potential suitability for applications within the food and pharmaceutical industries.

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超临界二氧化碳绿色溶剂萃取脆山羊：工艺优化、化学分析和生物活性评估

本研究首次利用超临界二氧化碳绿色溶剂从酥树蕨（Nepeta crispa）中提取化合物。该研究利用 RSM 分析了压力（15-25 兆帕）、温度（313-333 K）和助溶剂（0.5-3.5%）对萃取率的影响。通过铁还原抗氧化力（FRAP）测定法评估了精油的抗氧化活性，同时评估了精油对四种细菌菌株的抗菌特性。在最佳条件下（P= 25 兆帕、T= 313 K 和助溶剂= 3.5%），SCFE 法的最高产率为 1.812%。气相色谱-质谱分析表明，两种萃取方法中，酥树精油的主要成分都是 1,8-松油醇和 4aα,7α,7aα-epetalactone。N. crispa 的抗氧化和抗菌结果表明 SC-CO2 萃取法优于水蒸馏法。这些观察到的抗氧化和抗菌特性凸显了该植物在食品和制药行业应用的潜在适宜性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Supercritical Fluids 工程技术-工程：化工

CiteScore

7.60

自引率

10.30%

发文量

236

审稿时长

56 days

期刊介绍： The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.