Hydrogen sulfide enhances the disease resistance of ginger to rhizome rot during postharvest storage through modulation of antioxidant response and nitric oxide-mediated S-nitrosylaion

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-11-17 DOI:10.1016/j.postharvbio.2024.113321

Lingling Zhang , Xiuqiao Wu , Yue Zhong , Ying Yang , Shouhui Wei , Chong Sun , Lijuan Wei , Yiqing Liu

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引用次数: 0

Abstract

Postharvest pathogenic infestation leads to the quality deterioration in ginger industry. Hydrogen sulfide (H₂S), as an emerging potential postharvest protectant, could enhance disease resistance. This study investigated the antifungal role of H₂S against Fusarium solani during ginger postharvest storage. The results showed that H₂S restricted widespread infection by F. solani in gingers and have direct antimicrobial activity against F. solani in vitro, inhibiting mycelial growth and spore germination. H₂S improved endogenous H₂S accumulation, increased the activities of POD, CAT and SOD, and facilitated the removal of excess ROS. It also promoted the lignin, total phenolic and flavonoid contents, while boosting the activities of PAL, C4H and 4CL, and up-regulating the expression of ZoPAL, ZoC4H and Zo4CL. Moreover, H₂S increased endogenous NO levels through the NR and NOS pathways. Notably, the endogenous SNO content was increased, the GSNOR activity as well as expression of GSNOR were down-regulated by H₂S treatment. These effects were reversed by hypotaurine (HT), a scavenger of H₂S. Together, these results indicated that H₂S induces the disease resistance in postharvest ginger storage via enhancing antioxidant and defense capacity, regulating phenylpropane metabolism, inducing NO production and mediating NO-dependent S-nitrosylation modification. These results provide guidance for the application of H₂S during the storage of ginger.

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硫化氢通过调节抗氧化反应和一氧化氮介导的 S-亚硝基离子，增强生姜在收获后贮藏期间对根茎腐烂病的抗病性

收获后的病原体侵染导致生姜产业的质量下降。硫化氢（H2S）作为一种新兴的潜在采后保护剂，可以增强抗病性。本研究调查了 H2S 在生姜采后贮藏期间对禾谷镰刀菌的抗真菌作用。结果表明，H2S 限制了 F. solani 在生姜中的广泛感染，并在体外对 F. solani 具有直接抗菌活性，抑制菌丝生长和孢子萌发。H2S 改善了内源性 H2S 积累，提高了 POD、CAT 和 SOD 的活性，促进了过量 ROS 的清除。它还能提高木质素、总酚和类黄酮的含量，同时提高 PAL、C4H 和 4CL 的活性，并上调 ZoPAL、ZoC4H 和 Zo4CL 的表达。此外，H2S 还通过 NR 和 NOS 途径提高了内源性 NO 的水平。值得注意的是，H2S 处理增加了内源性 SNO 的含量，下调了 GSNOR 的活性和表达。H2S的清除剂低牛磺酸（HT）可逆转这些影响。这些结果表明，H2S 通过增强抗氧化和防御能力、调节苯丙氨酸代谢、诱导 NO 生成和介导 NO 依赖的 S-亚硝基化修饰，诱导收获后生姜贮藏过程中的抗病性。这些结果为在生姜贮藏过程中应用 H2S 提供了指导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.