Bioactivity-guided purification and characterization of antioxidant, anti-gout and anti-diabetic polyphenols from Panchkuta: A traditional food combination of (semi) arid regions

Food chemistry advances Pub Date : 2024-10-29 DOI:10.1016/j.focha.2024.100839

Tripti Joshi, Sonakshi Puri, PR Deepa, Pankaj Kumar Sharma

{"title":"Bioactivity-guided purification and characterization of antioxidant, anti-gout and anti-diabetic polyphenols from Panchkuta: A traditional food combination of (semi) arid regions","authors":"Tripti Joshi, Sonakshi Puri, PR Deepa, Pankaj Kumar Sharma","doi":"10.1016/j.focha.2024.100839","DOIUrl":null,"url":null,"abstract":"<div><div>Desert ecosystems have thrived in part because of the contribution of local traditions, along with conventional knowledge passed down through generations. <em>Panchkuta,</em> a combination of five plants, including <em>Prosopis cineraria, Cordia dichotoma, Capparis decidua, Acacia senegal</em>, and <em>Mangifera indica</em>, is a classic example of ethnic desert food and how communities utilize existing food resources to ensure nutritional security. In this study, we determined the total phenolic content, total flavonoid content, antioxidant, anti-gout, and anti-diabetic activities of crude and purified (using Amberlite XAD7HP and Sephadex LH-20) fractions of <em>Panchkuta</em>. Further, the phenolic compounds (quercetin, tiliroside, myricetin-3-O-galactoside, and others) responsible for these activities were identified by analytical techniques, including TLC, LC-MS, and HR-MS. The purified fraction showing maximum antioxidant activity in DPPH assay (Fraction 16, IC<sub>50</sub>–156.65 ± 12.6 μg/ml) was validated for its cytoprotective and anti-lipid peroxidative effects in the <em>in vitro</em> cell culture system (murine fibroblast cells). Fraction 9 showed the maximum anti-gout activity in xanthine oxidase assay (IC<sub>50</sub>- 80.59 ± 9.89 μg/ml) and fraction 11 showed the maximum anti-diabetic activity in α-amylase assay (IC<sub>50</sub> of 101.54 ± 14.7 μg/ml). The study reinforces the understanding of traditional knowledge, relevance to One Health, and authenticates the potential medicinal value of <em>Panchkuta</em> through scientific validation.</div></div>","PeriodicalId":73040,"journal":{"name":"Food chemistry advances","volume":"5 ","pages":"Article 100839"},"PeriodicalIF":0.0000,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food chemistry advances","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2772753X2400234X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Desert ecosystems have thrived in part because of the contribution of local traditions, along with conventional knowledge passed down through generations. Panchkuta, a combination of five plants, including Prosopis cineraria, Cordia dichotoma, Capparis decidua, Acacia senegal, and Mangifera indica, is a classic example of ethnic desert food and how communities utilize existing food resources to ensure nutritional security. In this study, we determined the total phenolic content, total flavonoid content, antioxidant, anti-gout, and anti-diabetic activities of crude and purified (using Amberlite XAD7HP and Sephadex LH-20) fractions of Panchkuta. Further, the phenolic compounds (quercetin, tiliroside, myricetin-3-O-galactoside, and others) responsible for these activities were identified by analytical techniques, including TLC, LC-MS, and HR-MS. The purified fraction showing maximum antioxidant activity in DPPH assay (Fraction 16, IC₅₀–156.65 ± 12.6 μg/ml) was validated for its cytoprotective and anti-lipid peroxidative effects in the in vitro cell culture system (murine fibroblast cells). Fraction 9 showed the maximum anti-gout activity in xanthine oxidase assay (IC₅₀- 80.59 ± 9.89 μg/ml) and fraction 11 showed the maximum anti-diabetic activity in α-amylase assay (IC₅₀ of 101.54 ± 14.7 μg/ml). The study reinforces the understanding of traditional knowledge, relevance to One Health, and authenticates the potential medicinal value of Panchkuta through scientific validation.

查看原文本刊更多论文

在生物活性指导下纯化和鉴定来自 Panchkuta 的抗氧化、抗痛风和抗糖尿病多酚：半干旱地区的传统食物组合

沙漠生态系统的繁荣部分归功于当地传统的贡献以及世代相传的传统知识。Panchkuta 是由五种植物（Prosopis cineraria、Cordia dichotoma、Capparis decidua、Acacia senegal 和 Mangifera indica）组合而成的，是沙漠民族食物以及社区如何利用现有食物资源确保营养安全的典型例子。在这项研究中，我们测定了板栗的粗制和纯化（使用 Amberlite XAD7HP 和 Sephadex LH-20）馏分的总酚含量、总黄酮含量、抗氧化、抗痛风和抗糖尿病活性。此外，还通过 TLC、LC-MS 和 HR-MS 等分析技术鉴定了具有这些活性的酚类化合物（槲皮素、桔梗苷、杨梅素-3-O-半乳糖苷等）。在 DPPH 试验中显示出最大抗氧化活性的纯化馏分（馏分 16，IC50-156.65 ± 12.6 μg/ml）在体外细胞培养系统（鼠成纤维细胞）中的细胞保护和抗脂质过氧化作用得到了验证。馏分 9 在黄嘌呤氧化酶测定中显示出最大的抗痛风活性（IC50- 80.59 ± 9.89 μg/ml），馏分 11 在 α 淀粉酶测定中显示出最大的抗糖尿病活性（IC50 为 101.54 ± 14.7 μg/ml）。这项研究加强了人们对传统知识的了解，与 "一体健康 "相关，并通过科学验证了 Panchkuta 的潜在药用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊