Antihyperglycemic potential of fermented Digitaria exilis polysaccharide partially substituted with Clendendrum volubile leaf extract

Ityotagher P. Aondoakaa , Gibson L. Arueya
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Abstract

Digitaria exilis and Clendendrum volubile are underutilized food resources common in Africa. Clendendrum volubile leaf has a history of being used in traditional medicine to treat or manage hyperglycemia. Despite Clendendrum volubile nutritive and pharmaceutical significance, no scientific study has explored its application in functional food development. In this study, the inhibitory activities of Clendendrum volubile leaf extract (CVLE) against α-amylase and α-glucosidase was determined. Fermented Digitaria exilis polysaccharide (FDEP) was partially substituted with CVLE at different percentages (0.8 % and 1.2 %) and its antihyperglycemic effect was investigated in alloxan-induced diabetic rat models. Results showed that the IC50 (CVLE extract concentration causing 50 % enzyme inhibition) for α-amylase and α-glucosidase were 330 μg/mL and 120 μg/mL, respectively, compared to acarbose, a standard inhibitor of starch-digesting enzymes, which had IC50 values of 370 μg/mL and 170 μg/mL for α-amylase and α-glucosidase, respectively. The animal study showed that FDEP containing 0.8 % CVLE lowered the blood glucose level of diabetic rats by 45.31 % after 24 days. Increasing CVLE concentration in the FDEP to 1.2 % not only reduced blood glucose by 72.97 % after 24 days, comparable to metformin's 74.29 % reduction, but also alleviated body weight loss and improved liver function by lowering alanine transaminase, aspartate transaminase, and alkaline phosphatase.
发酵地衣多糖的降血糖潜力--部分替代品为石斛叶提取物
Digitaria exilis 和 Clendrum volubile 是非洲常见的未充分利用的食物资源。在传统医学中,Clendrum volubile 叶有治疗或控制高血糖的历史。尽管 Clendrum volubile 具有重要的营养和药用价值,但目前还没有科学研究探索其在功能食品开发中的应用。本研究测定了卷柏叶提取物(CVLE)对α-淀粉酶和α-葡萄糖苷酶的抑制活性。用不同比例(0.8 % 和 1.2 %)的 CVLE 部分替代发酵地衣多糖(FDEP),并在阿脲诱导的糖尿病大鼠模型中研究其抗高血糖作用。结果表明,α-淀粉酶和α-葡萄糖苷酶的 IC50 值(CVLE 提取物浓度对酶的抑制率为 50%)分别为 330 μg/mL 和 120 μg/mL,而淀粉消化酶的标准抑制剂阿卡波糖对α-淀粉酶和α-葡萄糖苷酶的 IC50 值分别为 370 μg/mL 和 170 μg/mL。动物实验表明,含有 0.8 % CVLE 的 FDEP 在 24 天后可将糖尿病大鼠的血糖水平降低 45.31 %。将 FDEP 中的 CVLE 浓度提高到 1.2%,不仅能在 24 天后降低 72.97% 的血糖,与二甲双胍 74.29% 的降糖效果相当,还能减轻体重下降,并通过降低丙氨酸转氨酶、天门冬氨酸转氨酶和碱性磷酸酶来改善肝功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Food chemistry advances
Food chemistry advances Analytical Chemistry, Organic Chemistry, Chemistry (General), Molecular Biology
CiteScore
1.90
自引率
0.00%
发文量
0
审稿时长
99 days
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