Genetic diversity and population structure of Alternaria alternata: An endophytic fungus isolated from various hosts

IF 2.9 3区生物学 Q2 MYCOLOGY

Fungal biology Pub Date : 2024-11-09 DOI:10.1016/j.funbio.2024.11.005

Mostafa Ebadi , Ali Ebadi

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引用次数: 0

Abstract

Alternaria alternata is a significant fungal species that can function as both an endophytic fungus and a pathogen in various plant tissues. Unlike pathogenic fungi, endophytic fungi enhance the growth of host plants through different mechanisms. Studying the genetic diversity of endophytic fungi can provide insights into their co-evolution with plants. In this research, the genetic diversity of A. alternata from different hosts was examined using ten pairs of ISSR primers. Seven of the ten primers generated scorable polymorphic bands (total of 65 bands with an average of 9.2 bands per primer) for molecular analysis. Genetic diversity parameters revealed that isolates from Gundelia tournefortii exhibited the highest genetic diversity (Na, Ne, I, and He values of 1.55, 1.45, 0.35, and 0.24, respectively), while isolates from Tamarix ramosissima showed lower diversity (Na, Ne, I, and He values of 1.18, 1.13, 0.11, and 0.07, respectively). Cluster analysis grouped the isolates into four clusters based on Jaccard similarity matrix and UPGMA method. Principal coordinate analysis (PCOA) supported the cluster analysis findings. Analysis of molecular variance (AMOVA) indicated a high level of genetic differentiation within populations (72 %), with only 28 % of diversity between populations. The genetic structure assessment revealed a relatively strong genetic structure among populations, suggesting the presence of a hidden sexual cycle or mitotic recombination as factors contributing to the high genetic differentiation among A. alternata populations.

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Alternaria alternata 的遗传多样性和种群结构：从不同寄主中分离出的内生真菌

交替孢属真菌是一种重要的真菌物种，在各种植物组织中既可作为内生真菌，也可作为病原体。与病原真菌不同，内生真菌通过不同的机制促进宿主植物的生长。研究内生真菌的遗传多样性可以深入了解它们与植物的共同进化。本研究使用十对 ISSR 引物检测了不同寄主中交替花叶病毒的遗传多样性。十对引物中有七对产生了可扫描的多态性条带（共 65 条，平均每对引物 9.2 条），可用于分子分析。遗传多样性参数显示，Gundelia tournefortii 分离物的遗传多样性最高（Na、Ne、I 和 He 值分别为 1.55、1.45、0.35 和 0.24），而 Tamarix ramosissima 分离物的多样性较低（Na、Ne、I 和 He 值分别为 1.18、1.13、0.11 和 0.07）。聚类分析根据 Jaccard 相似性矩阵和 UPGMA 方法将分离物分为 4 个聚类。主坐标分析（PCOA）支持聚类分析结果。分子方差分析（AMOVA）表明，种群内的遗传分化程度较高（72%），种群间的多样性仅为 28%。遗传结构评估显示，种群间的遗传结构相对较强，这表明隐性有性生殖周期或有丝分裂重组的存在是造成交替花粉蚁种群间高度遗传分化的因素。

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来源期刊

Fungal biology MYCOLOGY-

CiteScore

5.80

自引率

4.00%

发文量

审稿时长

49 days

期刊介绍： Fungal Biology publishes original contributions in all fields of basic and applied research involving fungi and fungus-like organisms (including oomycetes and slime moulds). Areas of investigation include biodeterioration, biotechnology, cell and developmental biology, ecology, evolution, genetics, geomycology, medical mycology, mutualistic interactions (including lichens and mycorrhizas), physiology, plant pathology, secondary metabolites, and taxonomy and systematics. Submissions on experimental methods are also welcomed. Priority is given to contributions likely to be of interest to a wide international audience.