A novel lateral flow assay for lead ion detection based on G-quadruplex

IF 3.7 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2024-11-14 DOI:10.1016/j.bej.2024.109562

Linjiao Ren , Jingtong Sun , Shilin Ma , Diankang Wang , Rubin Qi , Pei Zhang , Qingfang Zhang , Zirui Qin , Liying Jiang

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引用次数: 0

Abstract

Lead ion residues pose potential health risks to humans. To develop a cost-effective and user-friendly portable lead ion detection method, a novel lateral flow test strip was designed and fabricated based on the G-quadruplex structure. Initially, G4 and its complementary strand antiG4 are in a double-stranded form in the assay solution. After adding lead ions, these ions compete with antiG4 to bind to G4 and form G-quadruplex structures, thus releasing a large number of antiG4 single strands. Through base pairing, one end of the antiG4 was linked to a recognition element containing gold nanoparticles, while the other end was captured by a test line probe, resulting in a red band on the test line. The color change of the test line was positively correlated with the concentration of lead ions. Results showed that by observing the color change of the test line under optimized experimental conditions, lead ion concentration could be detected with a visual detection limit of 20 nM. Quantitative analysis using ImageJ software indicated that the test strip had a linear detection range of 10–2000 nM, with a detection limit of 7.32 nM and significant specificity. The recovery rate in bottled drinking water ranged from 91.19 % to 126.04 %, providing a portable and simple new method for on-site detection of residual lead ions in water environments.

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基于 G 型四联体的新型横向流动铅离子检测分析法

铅离子残留对人体健康构成潜在风险。为了开发一种经济高效、操作简便的便携式铅离子检测方法，我们设计并制作了一种基于 G-四重结构的新型横向流动检测条。最初，G4 及其互补链 antiG4 以双链形式存在于检测溶液中。加入铅离子后，这些离子与 antiG4 竞争，与 G4 结合形成 G-四链结构，从而释放出大量 antiG4 单链。通过碱基配对，抗 G4 的一端与含有金纳米粒子的识别元件相连，而另一端则被检测线探针捕获，从而在检测线上形成一条红带。测试线的颜色变化与铅离子的浓度呈正相关。结果表明，在优化的实验条件下，通过观察检测线的颜色变化，可以检测到视觉检测限为 20 nM 的铅离子浓度。使用 ImageJ 软件进行的定量分析表明，该测试条的线性检测范围为 10-2000 nM，检测限为 7.32 nM，具有显著的特异性。在瓶装饮用水中的回收率为 91.19 % 至 126.04 %，为现场检测水环境中的残留铅离子提供了一种便携、简单的新方法。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.