Efficient and green preparation of baicalein from Radix scutellariae with deep eutectic solvent pretreated bioconversion of cellulose-based microreactor

IF 5.5 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Sustainable Chemistry and Pharmacy Pub Date : 2024-11-15 DOI:10.1016/j.scp.2024.101858

Shuang Jin , Yubin Ren , Yupeng Cheng , Weili Liu , Cailiang Peng , Bingyou Yang , Haixue Kuang , Yujie Fu , Chen Lv , Hongyao Cai

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Abstract

To enhance the bioconversion ratio, an efficient and green bioconversion method for the preparation of baicalein with a microreactor composed of edible microorganisms (Aspergillus oryzae and Yeast) treated with deep eutectic solvent co-immobilized on magnetic cellulose was investigated. Various types of deep eutectic solvents (DES) were screened to obtain a superior biocatalytic effect. NADES (CHCL/EG) harvested the maximum catalytic effect at 1.0 wt% under optimal conditions (concentration of bacterium 1.5 × 10⁷ (C × 10⁷ cells/mL), wet weight of microreactor 12 g, incubation temperature 33 °C and pH 5.0, liquid-solid ratio 30:1 (mL/g), incubation time 48 h), baicalein reached 66.36 mg/g, which increased 3.46-fold to that of normal sample after optimizing. DES could increase the permeability of the cell membrane. The bioconversion rate of baicalin reached 97.2%.

Moreover, IC₅₀ values of the extracts measured by the DPPH-radical scavenging test were 0.366 mg/mL (control sample 0.674 mg/mL). The residual activity of the microreactor was 80.2 % after using it 14 times. The experimental results showed that bioconversion is an effective method for preparing baicalein from the plants.

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利用基于纤维素的微反应器进行深共晶溶剂预处理生物转化，从黄芩中高效、绿色地制备黄芩素

为了提高生物转化率，研究人员利用磁性纤维素上共同固定的深共晶溶剂处理的可食用微生物（黑曲霉和酵母）组成的微反应器，研究了一种制备黄芩苷的高效绿色生物转化方法。为了获得更好的生物催化效果，对各种类型的深共晶溶剂（DES）进行了筛选。在最佳条件下（细菌浓度 1.5 × 107 (C × 107 cells/mL)，微反应器湿重 12 g，培养温度 33 °C 和 pH 5.0，液固比 30:1 (mL/g)，培养时间 48 h），NADES（CHCL/EG）在 1.0 wt%时获得了最大催化效果，黄芩素达到 66.36 mg/g，比优化后的普通样品提高了 3.46 倍。DES可增加细胞膜的通透性。黄芩苷的生物转化率达到 97.2%。此外，经 DPPH 自由基清除试验测定，提取物的 IC50 值为 0.366 mg/mL（对照样品为 0.674 mg/mL）。使用微反应器 14 次后，其剩余活性为 80.2%。实验结果表明，生物转化是一种从植物中制备黄芩苷的有效方法。

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来源期刊

Sustainable Chemistry and Pharmacy Environmental Science-Pollution

CiteScore

8.20

自引率

6.70%

发文量

274

审稿时长

37 days

期刊介绍： Sustainable Chemistry and Pharmacy publishes research that is related to chemistry, pharmacy and sustainability science in a forward oriented manner. It provides a unique forum for the publication of innovative research on the intersection and overlap of chemistry and pharmacy on the one hand and sustainability on the other hand. This includes contributions related to increasing sustainability of chemistry and pharmaceutical science and industries itself as well as their products in relation to the contribution of these to sustainability itself. As an interdisciplinary and transdisciplinary journal it addresses all sustainability related issues along the life cycle of chemical and pharmaceutical products form resource related topics until the end of life of products. This includes not only natural science based approaches and issues but also from humanities, social science and economics as far as they are dealing with sustainability related to chemistry and pharmacy. Sustainable Chemistry and Pharmacy aims at bridging between disciplines as well as developing and developed countries.