Identification of a novel GP60 subtype family of Cryptosporidium wrairi from capybaras (Hydrochoerus hydrochaeris) inhabiting urban areas in the state of Mato Grosso do Sul, Brazil

IF 1.4 Q3 PARASITOLOGY

Veterinary parasitology, regional studies and reports Pub Date : 2024-11-06 DOI:10.1016/j.vprsr.2024.101152

Camila Michele de Souza Hossotani , Fabio Eiji Fukumori , Flávia Maria de Almeida Moreira , Bárbara Braga Ferreira Marta , Débora Regina Romualdo da Silva , Marcelo Vasconcelos Meireles

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引用次数: 0

Abstract

Capybaras (Hydrochoerus hydrochaeris) are hosts for several parasites of public health importance, including Cryptosporidium spp. Therefore, this study aimed to perform the molecular characterization of Cryptosporidium spp. in fecal samples from capybaras inhabiting urban areas. We analyzed 401 fecal samples from capybaras in two municipalities of the state of Mato Grosso do Sul, Brazil. Fecal samples were purified using centrifugal sedimentation with ethyl acetate. They were then screened for Cryptosporidium spp. by malachite green negative staining and a nested PCR protocol targeting the 18S rRNA gene. Samples positive by microscopy or PCR were examined by PCR protocols targeting the actin, HSP-70, and GP60 genes. Amplicons from all PCR protocols were subjected to genetic sequencing. Microscopic screening and 18S rRNA gene-targeted PCR identified 0.25 % (1/401) and 0.5 % (2/401) of samples, respectively, as positive for Cryptosporidium spp. The genetic sequences of the 18S rRNA, HSP-70, actin, and GP60 genes showed genetic similarity to Cryptosporidium wrairi sequences of 99.07 %, 99.69 %, 99.57 %, and 91.51 %, respectively. Genetic sequencing and phylogenetic analyses identified the novel GP60 subtype family VIIbA13 of C. wrairi. In conclusion, we report in this study a low prevalence of the novel GP60 subtype family VIIbA13 of C. wrairi in free-living capybaras from urban areas of the state of Mato Grosso do Sul, Brazil. We propose that capybaras act as a novel host for C. wrairi.

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从巴西南马托格罗索州城市地区栖息的水豚中鉴定出新型 GP60 亚型隐孢子虫家族

水豚（Hydrochoerus hydrochaeris）是包括隐孢子虫属在内的几种对公共卫生具有重要意义的寄生虫的宿主，因此，本研究旨在对栖息在城市地区的水豚粪便样本中的隐孢子虫属进行分子鉴定。我们分析了巴西南马托格罗索州两个城市的 401 份毛冠鬣蜥粪便样本。粪便样本用乙酸乙酯离心沉淀法纯化。然后通过孔雀石绿阴性染色法和针对 18S rRNA 基因的巢式 PCR 方案筛查隐孢子虫属。对显微镜或 PCR 检测呈阳性的样本，采用针对肌动蛋白、HSP-70 和 GP60 基因的 PCR 方案进行检测。对所有 PCR 方案产生的扩增子进行基因测序。18S rRNA、HSP-70、肌动蛋白和 GP60 基因序列与隐孢子虫 wrairi 序列的遗传相似度分别为 99.07%、99.69%、99.57% 和 91.51%。基因测序和系统进化分析确定了 C. wrairi 的新型 GP60 亚型家族 VIIbA13。总之，我们在这项研究中报告说，在巴西南马托格罗索州城市地区自由生活的毛冠鼠中，C. wrairi 的新型 GP60 亚型家族 VIIbA13 的流行率很低。我们认为，水豚是 C. wrairi 的新型宿主。

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来源期刊

Veterinary parasitology, regional studies and reports Multiple-

CiteScore

2.90

自引率

7.10%

发文量

126

审稿时长

97 days

期刊介绍： Veterinary Parasitology: Regional Studies and Reports focuses on aspects of veterinary parasitology that are of regional concern, which is especially important in this era of climate change and the rapid and often unconstrained travel of people and animals. Relative to regions, this journal will accept papers of the highest quality dealing with all aspects of disease prevention, pathology, treatment, epidemiology, and control of parasites within the field of veterinary medicine. Also, case reports will be considered as they add to information related to local disease and its control; such papers must be concise and represent appropriate medical intervention. Papers on veterinary parasitology from wildlife species are acceptable, but only if they relate to the practice of veterinary medicine. Studies on vector-borne bacterial and viral agents are suitable, but only if the paper deals with vector transmission of these organisms to domesticated animals. Studies dealing with parasite control by means of natural products, both in vivo and in vitro, are more suited for one of the many journals that now specialize in papers of this type. However, due to the regional nature of much of this research, submissions may be considered based upon a case being made by the author(s) to the Editor. Circumstances relating to animal experimentation must meet the International Guiding Principles for Biomedical Research Involving Animals as issued by the Council for International Organizations of Medical Sciences (obtainable from: Executive Secretary C.I.O.M.S., c/o W.H.O., Via Appia, CH-1211 Geneva 27, Switzerland).