The establishment of reference intervals for the ClotPro thromboelastometry device in healthy dogs

IF 1.1 3区农林科学 Q3 VETERINARY SCIENCES

Journal of veterinary emergency and critical care Pub Date : 2024-11-18 DOI:10.1111/vec.13426

Samantha K. Day BVSc, MANZCVS, DACVECC, Katherine J. Nash BVSc, MS, MANZCVS, DACVECC, Mark J. Midwinter BMedSci, MB BS, MD, FRCS, CBE, Sarah L. Purcell BVSc, MANZCVS, MVECC, DACVECC, Wendy A. Goodwin PhD, BVSc, FANZCVS

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引用次数: 0

Abstract

Objective

To establish reference intervals using a new point-of-care thromboelastometry device in dogs for the extrinsically activated test (EX-test), intrinsically activated test (IN-test), fibrin polymerization test (FIB-test), ecarin test (ECA-test), and tissue plasminogen activator test (TPA-test) and to investigate the effects of storage time on the results.

Design

Prospective clinical study in 2022.

Setting

University teaching hospital.

Animals

Forty-eight healthy privately or university-owned dogs were prospectively enrolled and included on the basis of normal physical examination and normal baseline laboratory results (CBC, biochemistry profile, prothrombin time, and activated partial thromboplastin time [aPTT]).

Interventions

After a 30-minute storage time, the EX-test, IN-test, FIB-test, ECA-test, and TPA-test were performed on citrated blood samples. To determine the effect of storage time, 11 samples had the EX-test, FIB-test, and IN-test repeated 90 and 150 minutes after sample collection.

Measurements and Main Results

Ten thromboelastometry parameters were evaluated for each test. Reference intervals were calculated using the robust method for parametric data, and the robust Box–Cox transformed or nonparametric methods were used for nonparametric data. Increasing storage time resulted in more hypocoagulable tracings. A correlation was found between the IN-test and aPTT (r = 0.62, P < 0.0001). Other weak to moderate correlations were seen between thromboelastometry parameters and platelet count and hematocrit.

Conclusions and Clinical Importance

The development of reference intervals for the thromboelastometry device allows for the clinical use of this technology. Analyzing samples after a prolonged storage time of more than 30 minutes may result in erroneous results. Results may also be affected by an abnormal hematocrit or platelet count.

查看原文本刊更多论文

在健康狗身上建立 ClotPro 血栓弹性测量仪的参考区间。

目的使用一种新型护理点血栓弹性测定仪，为狗的外源性活化试验（EX-test）、内源性活化试验（IN-test）、纤维蛋白聚合试验（FIB-test）、依卡林试验（ECA-test）和组织纤溶酶原激活剂试验（TPA-test）建立参考区间，并研究储存时间对结果的影响：环境：大学教学医院：地点：大学教学医院：48只健康的私人或大学饲养的狗被纳入前瞻性研究，这些狗的体格检查和基线实验室结果（全血细胞计数、生化指标、凝血酶原时间和活化部分凝血活酶时间[aPTT]）均正常：储存 30 分钟后，对枸橼酸血样进行 EX 试验、IN 试验、FIB 试验、ECA 试验和 TPA 试验。为了确定储存时间的影响，在样本采集 90 分钟和 150 分钟后，对 11 份样本重复进行了 EX 测试、FIB 测试和 IN 测试：每项测试都评估了 10 个血栓弹性测量参数。参数数据采用稳健法计算参考区间，非参数数据采用稳健的箱-考克斯转换法或非参数法计算参考区间。储存时间越长，低凝描记越多。IN 测试和 aPTT 之间存在相关性（r = 0.62，P 结论和临床重要性）：血栓弹性测定仪参考区间的开发有助于该技术的临床应用。在样本储存时间超过 30 分钟后进行分析可能会导致错误的结果。血细胞比容或血小板计数异常也会影响结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of veterinary emergency and critical care 农林科学-兽医学

CiteScore

2.50

自引率

15.40%

发文量

121

审稿时长

18-36 weeks

期刊介绍： The Journal of Veterinary Emergency and Critical Care’s primary aim is to advance the international clinical standard of care for emergency/critical care patients of all species. The journal’s content is relevant to specialist and non-specialist veterinarians practicing emergency/critical care medicine. The journal achieves it aims by publishing descriptions of unique presentation or management; retrospective and prospective evaluations of prognosis, novel diagnosis, or therapy; translational basic science studies with clinical relevance; in depth reviews of pertinent topics; topical news and letters; and regular themed issues. The journal is the official publication of the Veterinary Emergency and Critical Care Society, the American College of Veterinary Emergency and Critical Care, the European Veterinary Emergency and Critical Care Society, and the European College of Veterinary Emergency and Critical Care. It is a bimonthly publication with international impact and adheres to currently accepted ethical standards.