Mithal K A Al-Hassani, Hadaf Mahdi Kadhim, Ahlam Ali Soghi Al-Galebi, Hasanain A J Gharban
{"title":"First molecular phylogeny of <i>Bartonella bovis</i> in Iraqi cattle.","authors":"Mithal K A Al-Hassani, Hadaf Mahdi Kadhim, Ahlam Ali Soghi Al-Galebi, Hasanain A J Gharban","doi":"10.5455/OVJ.2024.v14.i9.24","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong><i>Bartonella bovis</i> is one of the most neglected fastidious intracellular bacterial pathogens that infect a wide range of wild and domestic animals, including cattle.</p><p><strong>Aim: </strong>Detection of the prevalence of <i>B. bovis</i> in cattle using molecular assay, identification of the relationship of infection to animal risk factors (age and sex), and sequencing of <i>B. bovis</i> isolates to be analyzed phylogenetically and reported in the National Centre For Biotechnology Information (NCBI).</p><p><strong>Methods: </strong>A total of 290 cattle were selected randomly from different rural areas in Wasit province from September to December (2023), and subjected to collecting venous blood samples that were tested molecularly by the polymerase chain reaction (PCR). Phylogenetic analysis was conducted using the MEGA 7 Software.</p><p><strong>Results: </strong>Targeting the <i>16S rRNA</i> gene, 7.24% of the study cattle tested positive for <i>B. bovis</i>. In terms of age, positivity rates, odds ratios (ORs), and relative risks (RRs) were significantly higher in cattle older than 7 years compared to other age groups: under 1 year, 1-3 years, and 3-7 years. Regarding sex, there was no significant variation in positive rates between females (7.48%) and males (5.56%); however, the OR and RR values indicated that females (1.376 and 1.345, respectively) were at a higher risk of <i>B. bovis</i> infection than males (0.727 and 0.743, respectively). Phylogenetic tree analysis of five local <i>B. bovis</i> isolates demonstrated their identity with the France strain/isolate at total genetic identity ranging from 99.64% to 99.98%.</p><p><strong>Conclusion: </strong>To the best of our knowledge, this study represents the first molecular identification of the prevalence rate of <i>B. bovis</i> in cattle in Iraq using PCR assay and confirms phylogenetically the local <i>B. bovis</i> isolates in NCBI-BLAST. Therefore, additional information about the prevalence, genetic diversity in domestic and wild animals, and arthropod vectors is crucial for developing strategies for the prevention and control of <i>B. bovis</i> infections.</p>","PeriodicalId":19531,"journal":{"name":"Open Veterinary Journal","volume":"14 9","pages":"2361-2367"},"PeriodicalIF":0.9000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11563601/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Veterinary Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/OVJ.2024.v14.i9.24","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/30 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Bartonella bovis is one of the most neglected fastidious intracellular bacterial pathogens that infect a wide range of wild and domestic animals, including cattle.
Aim: Detection of the prevalence of B. bovis in cattle using molecular assay, identification of the relationship of infection to animal risk factors (age and sex), and sequencing of B. bovis isolates to be analyzed phylogenetically and reported in the National Centre For Biotechnology Information (NCBI).
Methods: A total of 290 cattle were selected randomly from different rural areas in Wasit province from September to December (2023), and subjected to collecting venous blood samples that were tested molecularly by the polymerase chain reaction (PCR). Phylogenetic analysis was conducted using the MEGA 7 Software.
Results: Targeting the 16S rRNA gene, 7.24% of the study cattle tested positive for B. bovis. In terms of age, positivity rates, odds ratios (ORs), and relative risks (RRs) were significantly higher in cattle older than 7 years compared to other age groups: under 1 year, 1-3 years, and 3-7 years. Regarding sex, there was no significant variation in positive rates between females (7.48%) and males (5.56%); however, the OR and RR values indicated that females (1.376 and 1.345, respectively) were at a higher risk of B. bovis infection than males (0.727 and 0.743, respectively). Phylogenetic tree analysis of five local B. bovis isolates demonstrated their identity with the France strain/isolate at total genetic identity ranging from 99.64% to 99.98%.
Conclusion: To the best of our knowledge, this study represents the first molecular identification of the prevalence rate of B. bovis in cattle in Iraq using PCR assay and confirms phylogenetically the local B. bovis isolates in NCBI-BLAST. Therefore, additional information about the prevalence, genetic diversity in domestic and wild animals, and arthropod vectors is crucial for developing strategies for the prevention and control of B. bovis infections.
期刊介绍：
Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
