Mapping of dwarfing gene and identification of mutant allele on plant height in wheat.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-11-15 eCollection Date: 2024-11-01 DOI:10.1007/s11032-024-01515-3

Xiaomei Xie, Yang Zhang, Le Xu, Hongchun Xiong, Yongdun Xie, Linshu Zhao, Jiayu Gu, Huiyuan Li, Jinfeng Zhang, Yuping Ding, Shirong Zhao, Huijun Guo, Luxiang Liu

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引用次数: 0

Abstract

Plant height is one of the most critical factors influencing wheat plant architecture, and the application of Green Revolution genes has led to a reduction in plant height and an increase in yield. Discovering new dwarfing genes and alleles can contribute to enhance the genetic diversity of wheat. Here we obtained an EMS induced dwarf wheat mutant je0166 with increased grain weight, which exhibited a reduction in plant height ranging from 46.47% to 49.40%, and its cell length was shorter. The mutant je0166 was sensitive to exogenous gibberellin, but its sensitivity was lower than that of its wild type. Genetic analysis on plant height and gene mapping located the target region to a 4.07 cM interval on chr. 4AL. Within this interval, we identified a co-segregated mutation in Rht-A1h, which is a novel allele of the Green Revolution gene Rht-A1. We also found large fragment inversions in the genetic map of the mutant. The novel allele diversifies natural allelic variations and could be utilized in future wheat improvement. Furthermore, we demonstrated that chemical mutagen treatment led to large fragment inversion.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-024-01515-3.

查看原文本刊更多论文

绘制矮化基因图谱并鉴定影响小麦株高的突变等位基因。

植株高度是影响小麦植株结构的最关键因素之一，绿色革命基因的应用导致了植株高度的降低和产量的增加。发现新的矮化基因和等位基因有助于提高小麦的遗传多样性。在此，我们获得了一个 EMS 诱导的矮秆小麦突变体 je0166，它的粒重增加，但株高降低了 46.47% 至 49.40%，细胞长度变短。突变体 je0166 对外源赤霉素敏感，但敏感性低于野生型。对植株高度和基因图谱的遗传分析将目标区域定位在 chr.4AL。在这个区间内，我们发现了 Rht-A1h 的共分离突变，这是绿色革命基因 Rht-A1 的一个新等位基因。我们还在突变体的基因图谱中发现了大片段倒位。该新型等位基因使天然等位基因变异多样化，可用于未来的小麦改良。此外，我们还证明了化学诱变剂处理会导致大片段反转：在线版本包含补充材料，可查阅 10.1007/s11032-024-01515-3。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.