Serum microRNA qPCR profiling and validation indicate upregulation of circulating miR-145-5p and miR-26a-5p in migraineurs.

IF 7.3 1区医学 Q1 CLINICAL NEUROLOGY

Journal of Headache and Pain Pub Date : 2024-11-18 DOI:10.1186/s10194-024-01908-x

Joanna Kordacka, Renata Gruszka, Magdalena Zakrzewska

{"title":"Serum microRNA qPCR profiling and validation indicate upregulation of circulating miR-145-5p and miR-26a-5p in migraineurs.","authors":"Joanna Kordacka, Renata Gruszka, Magdalena Zakrzewska","doi":"10.1186/s10194-024-01908-x","DOIUrl":null,"url":null,"abstract":"Background: In recent years, miRNAs found in biological fluids have gained interest as biomarkers of numerous conditions, including migraine. This study aimed to identify differences in the levels of circulating miRNAs in the serum of migraineurs as compared to healthy controls, as well as between patients with different types of migraine and during the ictal and nonictal phases of the condition.Methods: The screening phase of the study included serum from 13 migraine patients and 13 sex and age matched controls. A panel of 179 miRNAs was analysed using locked nucleic acid SYBR based qPCR. Based on statistical analysis (U Mann-Whitney test) and data from existing literature, nine miRNAs were selected for validation by TaqMan qPCR in an independent cohort of 26 migraineurs and eleven healthy controls. For comparison between the study and control group, U Mann-Whitney test was performed. The differences between patients with chronic and episodic migraine, migraine with and without aura and in ictal and nonictal phases were analysed with Kruskal-Wallis test. The results were corrected for multiple comparisons using Benjamini-Hochberg method. In all analysis p value ≤ 0,05 was considered as significant.Results: Two miRNAs, miR-145-5p and miR-26a-5p were significantly upregulated in serum of migraineurs compared to healthy controls. MiRNA-19a-3p was downregulated in patients currently experiencing migraine headache compared to those in the interictal period. No differences were found between patients with different migraine types.Conclusion: The results of our study add to the growing body of evidence for dysregulation of the circulating miRNA profile by migraine. They are further supported by previous reports on differential expression of miR-145-5p, miR-26a-5p and miR-19a-3p in migraineurs. However, more research on larger populations is needed to validate these findings, as well as elucidate the role of circulating miRNAs in the condition. Moreover, to wholly explore the biomarker potential of miRNAs, migraine patients should not only be compared to healthy controls but also to populations with different headache disorders.","PeriodicalId":16013,"journal":{"name":"Journal of Headache and Pain","volume":"25 1","pages":"198"},"PeriodicalIF":7.3000,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11571994/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Headache and Pain","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s10194-024-01908-x","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: In recent years, miRNAs found in biological fluids have gained interest as biomarkers of numerous conditions, including migraine. This study aimed to identify differences in the levels of circulating miRNAs in the serum of migraineurs as compared to healthy controls, as well as between patients with different types of migraine and during the ictal and nonictal phases of the condition.

Methods: The screening phase of the study included serum from 13 migraine patients and 13 sex and age matched controls. A panel of 179 miRNAs was analysed using locked nucleic acid SYBR based qPCR. Based on statistical analysis (U Mann-Whitney test) and data from existing literature, nine miRNAs were selected for validation by TaqMan qPCR in an independent cohort of 26 migraineurs and eleven healthy controls. For comparison between the study and control group, U Mann-Whitney test was performed. The differences between patients with chronic and episodic migraine, migraine with and without aura and in ictal and nonictal phases were analysed with Kruskal-Wallis test. The results were corrected for multiple comparisons using Benjamini-Hochberg method. In all analysis p value ≤ 0,05 was considered as significant.

Results: Two miRNAs, miR-145-5p and miR-26a-5p were significantly upregulated in serum of migraineurs compared to healthy controls. MiRNA-19a-3p was downregulated in patients currently experiencing migraine headache compared to those in the interictal period. No differences were found between patients with different migraine types.

Conclusion: The results of our study add to the growing body of evidence for dysregulation of the circulating miRNA profile by migraine. They are further supported by previous reports on differential expression of miR-145-5p, miR-26a-5p and miR-19a-3p in migraineurs. However, more research on larger populations is needed to validate these findings, as well as elucidate the role of circulating miRNAs in the condition. Moreover, to wholly explore the biomarker potential of miRNAs, migraine patients should not only be compared to healthy controls but also to populations with different headache disorders.

查看原文本刊更多论文

血清 microRNA qPCR 分析和验证表明，偏头痛患者循环 miR-145-5p 和 miR-26a-5p 上调。

背景：近年来，生物体液中发现的 miRNA 作为包括偏头痛在内的多种疾病的生物标志物引起了人们的兴趣。本研究旨在确定偏头痛患者血清中循环 miRNA 水平与健康对照组的差异，以及不同类型偏头痛患者之间、偏头痛发作期与非发作期之间的差异：研究的筛选阶段包括 13 名偏头痛患者和 13 名性别与年龄匹配的对照组的血清。使用基于锁定核酸 SYBR 的 qPCR 分析了 179 个 miRNA。根据统计分析（U Mann-Whitney 检验）和现有文献中的数据，在 26 名偏头痛患者和 11 名健康对照者组成的独立队列中选择了 9 个 miRNA 进行 TaqMan qPCR 验证。研究组与对照组之间的比较采用了 U Mann-Whitney 检验。慢性偏头痛患者与发作性偏头痛患者、有先兆偏头痛患者与无先兆偏头痛患者、发作期偏头痛患者与非发作期偏头痛患者之间的差异采用 Kruskal-Wallis 检验进行分析。使用本杰明-霍奇伯格法对结果进行多重比较校正。在所有分析中，P值小于0.05为显著：结果：与健康对照组相比，偏头痛患者血清中的两个 miRNA（miR-145-5p 和 miR-26a-5p）明显上调。与发作间期的偏头痛患者相比，偏头痛患者血清中的 miRNA-19a-3p 下调。不同类型偏头痛患者之间没有发现差异：我们的研究结果为偏头痛引起的循环 miRNA 配置失调提供了更多证据。以前关于偏头痛患者体内 miR-145-5p、miR-26a-5p 和 miR-19a-3p 不同表达的报道进一步支持了这些研究结果。然而，要验证这些发现，并阐明循环 miRNA 在偏头痛中的作用，还需要对更多人群进行更多研究。此外，要全面探索 miRNA 的生物标记潜力，偏头痛患者不仅要与健康对照组进行比较，还要与不同头痛疾病的人群进行比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Headache and Pain 医学-临床神经学

CiteScore

11.80

自引率

13.50%

发文量

143

审稿时长

6-12 weeks

期刊介绍： The Journal of Headache and Pain, a peer-reviewed open-access journal published under the BMC brand, a part of Springer Nature, is dedicated to researchers engaged in all facets of headache and related pain syndromes. It encompasses epidemiology, public health, basic science, translational medicine, clinical trials, and real-world data. With a multidisciplinary approach, The Journal of Headache and Pain addresses headache medicine and related pain syndromes across all medical disciplines. It particularly encourages submissions in clinical, translational, and basic science fields, focusing on pain management, genetics, neurology, and internal medicine. The journal publishes research articles, reviews, letters to the Editor, as well as consensus articles and guidelines, aimed at promoting best practices in managing patients with headaches and related pain.