Virulence and molecular epidemiological analysis of three human blood-borne Streptococcus suis.

IF 3.4 3区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Heliyon Pub Date : 2024-10-30 eCollection Date: 2024-11-15 DOI:10.1016/j.heliyon.2024.e39978

Qianying Zhu, Fei Xia, Zhe Chen, Sen Lin, Qing Zhang, Bingru Xue, Weisi Dai

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引用次数: 0

Abstract

Objective: To understand the virulence genes and molecular epidemiological characteristics of human-infected strains of Streptococcus suis in Rui'an, Zhejiang Province, from 2021 to 2022, and to provide a scientific basis for diagnosis, treatment, prevention, and control.

Methods: Three blood-borne strains of Streptococcus suis were analysed by morphological observation, identification, and drug sensitivity tests. We performed polymerase chain reaction (PCR) amplification of their main seven virulence factors and housekeeping genes. This was followed by virulence analysis and multilocus sequence typing. We analysed their relationships with local pathogens from previous years.

Results: Three Streptococcus suis strains were isolated from the blood samples of three patients. From these, the virulence genotypes demonstrated that the two strains were orf2+ and ef+/orf2+/sly+, respectively. The Multilocus sequence typing (MLST) typing results demonstrated that the two strains were ST25 and ST7, respectively.

Conclusion: The first isolation of ST25 Streptococcus suis in Rui'an was presumed to have a close affinity with the endemic strain in North America. The other strain was an ST7 clone, consistent with the endemic strain in Sichuan, and which may have originated from Sichuan. Virulence genotype analysis demonstrated that different virulence genes of the pathogens resulted in different clinical manifestations.

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三种人类血源性猪链球菌的毒性和分子流行病学分析。

目的了解2021-2022年浙江省瑞安市人感染猪链球菌菌株的毒力基因和分子流行病学特征，为诊断、治疗和防控提供科学依据：方法：通过形态学观察、鉴定和药敏试验，对 3 株血源性猪链球菌进行分析。我们对其主要的七种毒力因子和看家基因进行了聚合酶链式反应（PCR）扩增。然后进行毒力分析和多焦点序列分型。我们分析了它们与往年本地病原体的关系：结果：从三名患者的血液样本中分离出三株猪链球菌。结果：从三名患者的血液样本中分离出三株猪链球菌，其中两株的毒力基因型分别为orf2+和ef+/orf2+/sly+。多焦点序列分型（MLST）结果表明，这两株菌株分别为 ST25 和 ST7：结论：在瑞安首次分离到的 ST25 猪链球菌推测与北美流行株有密切关系。结论：在瑞安首次分离到的 ST25 猪链球菌推测与北美流行株亲缘关系密切，另一株为 ST7 克隆株，与四川流行株一致，可能来自四川。毒力基因型分析表明，病原体的不同毒力基因导致了不同的临床表现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Heliyon MULTIDISCIPLINARY SCIENCES-

CiteScore

4.50

自引率

2.50%

发文量

2793

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