Development of polyclonal chicken egg yolk immunoglobulin Y (IgY) antibodies targeting SARS-CoV-2 multi-epitope antigen

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2024-11-17 DOI:10.1016/j.jviromet.2024.115062

Azzania Fibriani, Katerina Naisanu, Nicholas Yamahoki, Denti Rizki Kinanti

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引用次数: 0

Abstract

Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) is the primary cause of the Coronavirus disease 2019 (COVID-19) pandemic, which affects millions of people worldwide with high levels of infectivity and mortality. However, the antibodies developed for COVID-19 research and diagnostics are still limited. Therefore, in this study, we developed polyclonal immunoglobulin (IgY) antibodies from chicken egg yolk targeting multi-epitope antigen of SARS-CoV-2. After immunizing hens with a SARS-CoV-2 multi-epitope peptide, IgY antibodies were isolated from chicken eggs and further characterized using SDS-PAGE and ELISA. The results showed that the IgY antibodies were successfully isolated from egg yolks. The sandwich ELISA results demonstrated that the isolated IgYs could bind to SARS-CoV-2 antigens, both the multi-epitope peptide and the trimeric Spike. Furthermore, the developed polyclonal antibodies could recognize SARS-CoV-2 in human nasopharyngeal swab samples, even at the lowest concentration (dilution at 1:10000). Thus, it can be concluded that the developed polyclonal IgYs were successfully produced and have the potential to be applied in the development of COVID-19 diagnostics.

查看原文本刊更多论文

针对 SARS-CoV-2 多表位抗原的多克隆鸡卵黄免疫球蛋白 Y (IgY) 抗体的开发。

严重急性呼吸系统综合征-冠状病毒-2（SARS-CoV-2）是冠状病毒病 2019（COVID-19）大流行的主要原因，它影响着全球数百万人，感染率和死亡率都很高。然而，为 COVID-19 研究和诊断开发的抗体仍然有限。因此，在本研究中，我们从鸡卵黄中开发了针对 SARS-CoV-2 多表位抗原的多克隆免疫球蛋白（IgY）抗体。用 SARS-CoV-2 多表位肽免疫母鸡后，我们从鸡卵中分离出了 IgY 抗体，并用 SDS-PAGE 和 ELISA 对其进行了进一步鉴定。结果显示，从蛋黄中成功分离出了 IgY 抗体。夹心酶联免疫吸附试验结果表明，分离出的 IgYs 能与 SARS-CoV-2 抗原结合，包括多表位肽和三聚 Spike。此外，即使在最低浓度（1:10000 稀释度）下，所开发的多克隆抗体也能识别人类鼻咽拭子样本中的 SARS-CoV-2。因此，可以得出结论，研制成功的多克隆 IgYs 有潜力应用于 COVID-19 诊断方法的开发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.