Correlation of different HPV genotype viral loads and cervical lesions: A retrospective analysis of 1585 cases.

IF 2.6 3区医学 Q3 ONCOLOGY

Cancer Cytopathology Pub Date : 2024-11-18 DOI:10.1002/cncy.22920

Yilu Zhou, Jiaxin Liu, Shuai Chen, Xianzhen Xin, Mohan Xiao, Xian Qiang, Lina Zhang

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引用次数: 0

Abstract

Background: To reduce unnecessary examinations and treatments, an effective detection method for differentiating human papillomavirus (HPV)-positive patients is urgently needed. This study aimed to explore the differences in HPV viral loads across various cervical lesions and identify the optimal cutoff value for high-grade squamous intraepithelial lesions (HSILs).

Methods: This retrospective study included patients with varying degrees of cervical lesions admitted to a hospital between January 1, 2023, and March 1, 2024. The HPV genotype and viral load were determined using BioPerfectus multiplex real-time assay. The differences in HPV genotype viral loads among cervical lesion classifications were analyzed to identify the most applicable type of viral load.

Results: The viral loads of HPV16, HPV31, HPV33, HPV35, and HPV58 were significantly associated with the grade of cervical lesions (p < .05), with the HPV16 group exhibiting the strongest correlation (p < .01). The HPV16 viral load demonstrated good sensitivity (Se) and specificity (Sp) for predicting HSIL (Se = 81.52%, Sp = 64.13%). The three most prevalent HPV genotypes associated with negative, low-grade squamous intraepithelial lesions (LSILs) and HSILs were HPV16, HPV52, and HPV58. HPV33 exhibited the highest prevalence of HSILs, followed by HPV16.

Conclusions: High-risk HPV viral load is associated with cervical lesion classification. HPV16 viral load can effectively differentiate HSIL from LSIL with good Se and Sp.

查看原文本刊更多论文

不同 HPV 基因型病毒载量与宫颈病变的相关性：对 1585 例病例的回顾性分析。

背景：为了减少不必要的检查和治疗，迫切需要一种有效的检测方法来区分人乳头瘤病毒（HPV）阳性患者。本研究旨在探讨不同宫颈病变中 HPV 病毒载量的差异，并确定高级别鳞状上皮内病变（HSIL）的最佳临界值：这项回顾性研究纳入了2023年1月1日至2024年3月1日期间入住医院的不同程度宫颈病变患者。HPV基因型和病毒载量采用BioPerfectus多重实时检测法进行测定。分析了不同宫颈病变分类中 HPV 基因型病毒载量的差异，以确定最适用的病毒载量类型：结果：HPV16、HPV31、HPV33、HPV35 和 HPV58 的病毒载量与宫颈病变的等级显著相关（p 结论：HPV16、HPV31、HPV33、HPV35 和 HPV58 的病毒载量与宫颈病变的等级显著相关：高危型 HPV 病毒载量与宫颈病变分级相关。HPV16病毒载量能有效区分HSIL和LSIL，且Se和Sp良好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Cytopathology 医学-病理学

CiteScore

7.00

自引率

17.60%

发文量

130

审稿时长

1 months

期刊介绍： Cancer Cytopathology provides a unique forum for interaction and dissemination of original research and educational information relevant to the practice of cytopathology and its related oncologic disciplines. The journal strives to have a positive effect on cancer prevention, early detection, diagnosis, and cure by the publication of high-quality content. The mission of Cancer Cytopathology is to present and inform readers of new applications, technological advances, cutting-edge research, novel applications of molecular techniques, and relevant review articles related to cytopathology.