The Hcp2b of APEC induces mitochondrial damage in chicken DF-1 cells.

IF 2.5 2区农林科学 Q1 VETERINARY SCIENCES

Avian Pathology Pub Date : 2024-12-19 DOI:10.1080/03079457.2024.2431803

Liting Lu, Zhao Qi, Haiyang Wang, Zhe Chen, Zichao Song, Ziqi Li, Xiaoru Wang, Bingyu Zhao, Xiyang Wei, Ying Shao, Zhenyu Wang, Jian Tu, Xiangjun Song

引用次数: 0

Abstract

The haemolysin co-regulatory protein (Hcp) plays a significant role in the pathogenicity of avian pathogenic Escherichia coli (APEC) as an effector protein of the type VI secretion system (T6SS) to the host. Meanwhile, mitochondria in the host are the target of effector proteins of various secretion systems. Here, we explored the effects of APEC effector Hcp2b on the mitochondria of DF-1 cells and found that Hcp2b results in damage in mitochondria. Next, 68 target proteins in DF-1 cell lysates were identified that interacted with Hcp2b by streptavidin-biotin pull-down assay combined with LC-MS/MS, among which ADP/ATP transporter carrier (SLC25A4) is a mitochondria-associated protein; protein docking analysis showed that Hcp2b binds well to SLC25A4. Therefore, we hypothesize that the Hcp2b contributes to mitochondrial damage in DF-1 cells through interaction with the SLC25A4.

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APEC 的 Hcp2b 可诱导鸡 DF-1 细胞线粒体损伤。

摘要溶血素协同调控蛋白（Hcp）作为 VI 型分泌系统（T6SS）的效应蛋白，在禽致病性大肠杆菌（APEC）对宿主的致病性中发挥着重要作用。同时，宿主的线粒体也成为各种分泌系统效应蛋白的靶标。在此，我们希望探讨 APEC 效应蛋白 Hcp2b 对 DF-1 细胞线粒体的影响，结果发现 Hcp2b 会导致线粒体损伤。接着，通过链霉亲和素-生物素牵引试验结合LC-MS/MS，在DF-1细胞裂解液中发现了68个与Hcp2b相互作用的靶蛋白，其中ADP/ATP转运载体（SLC25A4）是线粒体相关蛋白，蛋白对接分析表明Hcp2b与SLC25A4结合良好。因此，我们推测 Hcp2b 是通过与 SLC25A4 相互作用而导致 DF-1 细胞线粒体损伤的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Avian Pathology 农林科学-兽医学

CiteScore

4.50

自引率

10.70%

发文量

审稿时长

1 months

期刊介绍： Avian Pathology is the official journal of the World Veterinary Poultry Association and, since its first publication in 1972, has been a leading international journal for poultry disease scientists. It publishes material relevant to the entire field of infectious and non-infectious diseases of poultry and other birds. Accepted manuscripts will contribute novel data of interest to an international readership and will add significantly to knowledge and understanding of diseases, old or new. Subject areas include pathology, diagnosis, detection and characterisation of pathogens, infections of possible zoonotic importance, epidemiology, innate and immune responses, vaccines, gene sequences, genetics in relation to disease and physiological and biochemical changes in response to disease. First and subsequent reports of well-recognized diseases within a country are not acceptable unless they also include substantial new information about the disease or pathogen. Manuscripts on wild or pet birds should describe disease or pathogens in a significant number of birds, recognizing/suggesting serious potential impact on that species or that the disease or pathogen is of demonstrable relevance to poultry. Manuscripts on food-borne microorganisms acquired during or after processing, and those that catalogue the occurrence or properties of microorganisms, are unlikely to be considered for publication in the absence of data linking them to avian disease.