Effects of different extraction temperatures on the structural characteristics and antioxidant activity of polysaccharides from dandelion leaves.

IF 7.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2024-11-15 DOI:10.1016/j.ijbiomac.2024.137726

Pei Chen, Ee Sang, Huanhuan Chen, Qi Meng, Huiping Liu

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引用次数: 0

Abstract

Dandelion polysaccharides contribute to a variety of biological activities. This study evaluated the effect of different extraction temperatures (4 °C and 80 °C) on the structural characteristics and antioxidant activity of dandelion leaf polysaccharides (DLP). The findings demonstrated that the extraction efficiency improved at the higher temperature, while molecular weight exist a trend of degradation with increasing extraction temperature. Ion chromatography (IC) analysis indicated that the polysaccharides DLP4 and DLP80 were structurally complex heteropolysaccharides mainly composed of galactose, arabinose, glucose and mannose, with galactose and arabinose dominating. FT-IR and methylation analysis revealed that DLP4 and DLP80 had similar chemical structures and branches. DLP4 contained a higher amount of 6-Galactose. Microstructure analysis showed that heat treatment caused conformational changes in DLP4 and DLP80. Both had excellent free radical scavenging ability including DPPH·, ABTS^·+, OH· and reducing power. The Reactive Oxygen Species assay indicated that the protective effect of DLP4 against H₂O₂-induced oxidative damage in vitro was stronger than that of DLP80. Superoxide dismutase (SOD) and malondialdehyde (MDA) measurements also confirmed that the antioxidant effect of DLP4 was more prominent. Overall, low temperature extracted DLP can be used as an antioxidant in the areas of food, medicine and biomaterials.

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不同提取温度对蒲公英叶多糖结构特征和抗氧化活性的影响

蒲公英多糖具有多种生物活性。本研究评估了不同提取温度（4 °C和80 °C）对蒲公英叶多糖（DLP）结构特征和抗氧化活性的影响。研究结果表明，温度越高，提取效率越高，而分子量则随着提取温度的升高呈下降趋势。离子色谱（IC）分析表明，多糖 DLP4 和 DLP80 是结构复杂的杂多糖，主要由半乳糖、阿拉伯糖、葡萄糖和甘露糖组成，其中以半乳糖和阿拉伯糖为主。傅立叶变换红外光谱和甲基化分析表明，DLP4 和 DLP80 具有相似的化学结构和分支。DLP4 含有较多的 6-半乳糖。显微结构分析表明，热处理会导致 DLP4 和 DLP80 的构象发生变化。两者都具有出色的自由基清除能力，包括 DPPH-、ABTS-+、OH- 和还原力。活性氧检测表明，DLP4 对 H2O2 诱导的体外氧化损伤的保护作用强于 DLP80。超氧化物歧化酶（SOD）和丙二醛（MDA）的测定也证实，DLP4 的抗氧化作用更为突出。总之，低温提取的 DLP 可用作食品、医药和生物材料领域的抗氧化剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.