RNAi-based transgenic maize to control double-spotted leaf beetle (Monolepta hieroglyphica).

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-11-18 DOI:10.1002/ps.8543

Fahao Wang, Kangdi Zhou, Yifan Yu, Luyao Peng, Yuxuan Ye, Chaoyang Lin, Chao Xu, Zhicheng Shen

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引用次数: 0

Abstract

Background: The double-spotted leaf beetle (DLB), Monolepta hieroglyphica, is becoming a significant corn pest in China. It mainly attacks corn silk and developing kernels during the adult stage and is causing significant corn yield loss in north-eastern China. The damage caused by DLB is expected to worsen as pesticide usage is likely to decrease along with the upcoming commercial planting of transgenic lepidopteran-resistant maize in China. Therefore, it is highly desirable to develop transgenic corn for DLB resistance.

Results: Three target genes, MhSsj1, MhSnf7 and MhSec23A were cloned from DLB by their sequence similarity to their corresponding homologous genes known for their effectiveness as RNA interference (RNAi) targets in western corn rootworm (WCR, Diabrotica virgifera virgifera). Injection of the double-stranded RNAs (dsRNAs) of MhSsj1, MhSnf7 and MhSec23A to DLB adults was highly effective to suppress the messenger RNAs (mRNAs) of these genes and resulted in high mortality. Furthermore, a synergistic effect was observed among the dsRNAs of these three target genes. Transgenic maize plants simultaneously transcribing dsRNAs of any two of the three target genes were found to be highly resistant to DLB adults, showcasing the potential of utilizing RNAi-based strategy for transgenic DLB control.

Conclusion: MhSsj1, MhSnf7 and MhSec23A are effective RNAi target genes and transgenic corn based on suppression of these genes by RNAi are effective for controlling adult DLB. © 2024 Society of Chemical Industry.

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基于 RNAi 的转基因玉米控制双斑叶甲（Monolepta hieroglyphica）。

背景：双斑叶甲（DLB）正在成为中国玉米的一种重要害虫。它主要在成虫期危害玉米丝和正在发育的籽粒，在中国东北地区造成严重的玉米减产。随着转基因抗鳞翅目害虫玉米即将在中国商业化种植，农药用量可能会减少，预计 DLB 造成的危害将进一步加剧。因此，开发抗鳞翅目害虫的转基因玉米是非常可取的：结果：从 DLB 中克隆了三个靶基因 MhSsj1、MhSnf7 和 MhSec23A，因为它们与相应的同源基因序列相似，而这些同源基因是西部玉米根虫（WCR，Diabrotica virgifera virgifera）有效的 RNA 干扰（RNAi）靶基因。向DLB成虫注射MhSsj1、MhSnf7和MhSec23A的双链RNA（dsRNA）可高效抑制这些基因的信使RNA（mRNA），并导致高死亡率。此外，还观察到这三个靶基因的 dsRNA 之间存在协同效应。同时转录这三个靶基因中任何两个基因的dsRNA的转基因玉米植株对DLB成虫具有很强的抗性，展示了利用基于RNAi的转基因DLB控制策略的潜力：结论：MhSsj1、MhSnf7 和 MhSec23A 是有效的 RNAi 靶基因，基于 RNAi 抑制这些基因的转基因玉米可有效控制 DLB 成虫。© 2024 化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.