RNA interference-mediated targeting of monooxygenase SsMNO1 for controlling Sclerotinia stem rot caused by Sclerotinia sclerotiorum.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-11-18 DOI:10.1002/ps.8546

Lili Han, Yali Li, Zihong Yuan, Jing Wang, Binnian Tian, Anfei Fang, Yuheng Yang, Chaowei Bi, Yang Yu

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Abstract

Background: Sclerotinia sclerotiorum is a devastating fungal pathogen that poses a threat to a variety of economically important crops. Owing to the lack of highly resistant cultivars and the prolonged survival of sclerotia, effective control of Sclerotinia diseases remains challenging. RNA interference (RNAi) agents targeting essential active transcripts of genes associated with the development and virulence of pathogens are a valuable and promising disease control method.

Results: Our finding suggested that a flavin adenine dinucleotide (FAD)-dependent monooxygenase gene SsMNO1 plays pivotal roles in the hyphal growth, sclerotial development, and virulence of S. sclerotiorum, rendering it a potential target for RNAi-mediated management of S. sclerotiorum. The external application of double-stranded RNA (dsRNA) targeting SsMNO1 inhibited sclerotial development in artificial media and plant tissues. Furthermore, dsRNA significantly reduced the hyphal virulence of S. sclerotiorum in host plants by interfering with SsMNO1 expression. The inhibitory activity persisted for over 1 week on the surface of Brassica napus. Artificial small interfering RNA (siRNA) targeting SsMNO1 also exhibited inhibitory effects. Transgenic Arabidopsis thaliana plants expressing SsMNO1 hairpin RNAi constructs showed increased resistance to S. sclerotiorum infection. Notably, the total RNA extracts from SsMNO1-RNAi plants also reduced the hyphal virulence in Brassica napus.

Conclusions: Therefore, RNAi agents targeting SsMNO1 have dual effects on sclerotial development and hyphal virulence, rendering it an ideal target for controlling diseases caused by S. sclerotiorum. © 2024 Society of Chemical Industry.

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以 RNA 干扰为介导的单氧化酶 SsMNO1 为靶标，控制由 Sclerotinia sclerotiorum 引起的茎腐病。

背景：硬孢菌是一种毁灭性真菌病原体，对多种具有重要经济价值的作物构成威胁。由于缺乏高抗性的栽培品种以及硬孢菌的长期存活，有效控制硬孢菌病仍然具有挑战性。针对与病原体发育和毒力相关的重要活性基因转录本的 RNA 干扰（RNAi）制剂是一种有价值且有前景的病害控制方法：结果：我们的研究结果表明，依赖于黄素腺嘌呤二核苷酸（FAD）的单氧化酶基因 SsMNO1 在硬核菌的菌丝生长、硬壳发育和毒力方面起着关键作用，因此是 RNAi 介导的硬核菌控制的潜在靶标。外部应用靶向 SsMNO1 的双链 RNA（dsRNA）可抑制人工培养基和植物组织中的硬菌发育。此外，dsRNA 通过干扰 SsMNO1 的表达，大大降低了 S. sclerotiorum 在寄主植物中的芽孢毒力。这种抑制活性在甘蓝菜表面可持续一周以上。针对 SsMNO1 的人工小干扰 RNA（siRNA）也表现出抑制作用。表达 SsMNO1 发夹式 RNAi 构建体的转基因拟南芥植株对 S. sclerotiorum 感染的抗性增强。值得注意的是，SsMNO1-RNAi 植物的总 RNA 提取物也降低了甘蓝型油菜的头状病毒力：因此，以 SsMNO1 为靶标的 RNAi 制剂对硬核菌的发育和球茎毒力具有双重影响，使其成为控制由硬核菌引起的病害的理想靶标。© 2024 化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.