Comparison of zooplankton assimilation of different carbon sources and fatty acids in a eutrophic lake and its restored basins

Abstract

The ecological restoration of eutrophic lakes significantly influences the food composition of zooplankton. Zooplankton serve as the principal trophic link, transferring energy from phytoplankton to fish. Understanding the alterations in zooplankton carbon source compositions following ecological restoration and the seasonal variations in this relationship is crucial. This study employs stable carbon isotope (δ¹³C) and fatty acid (FA) analyses to investigate the seasonal changes in carbon source contributions to zooplankton between the restored and unrestored segments of Lake Xuanwu. Results from FA analysis reveal higher proportions of algal dietary markers in zooplankton FAs in both segments during spring and autumn. Summer exhibits a shift with zooplankton utilizing more bacterial FAs in the restored part and more algal FAs in the unrestored part. While approaching winter, zooplankton in the restored part consume more algal FAs but less in the unrestored part. Zooplankton FAs enriched in δ¹³C are associated with assimilation of more terrestrial carbon, contrasting with depleted δ¹³C when zooplankton relies more on phytoplankton. Isotope mixing models indicate a substantial contribution of terrestrial carbon to zooplankton carbon sources, especially in autumn (42.3% unrestored, 51.2% restored) and winter (41.4% unrestored, 36.8% restored), while phytoplankton has a higher contribution in summer (34.5% restored, 46.9% unrestored). These findings contribute to a comprehensive understanding of carbon cycling variations in food webs between eutrophic lakes and ecologically restored lakes.