Self-healing Ppy-hydrogel promotes diabetic skin wound healing through enhanced sterilization and macrophage orchestration triggered by NIR

IF 12.8 1区医学 Q1 ENGINEERING, BIOMEDICAL

Biomaterials Pub Date : 2024-11-12 DOI:10.1016/j.biomaterials.2024.122964

Zhuangzhuang Chu , Xingdan Liu , Tong Zhao , Dongya Jiang , Jing Zhao , Xiaohua Dong , Kelvin W.K. Yeung , Xuanyong Liu , Yun Liao , Liping Ouyang

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引用次数: 0

Abstract

Non-healing diabetic foot ulcers are the knotty public health issue due to the uncontrolled bacterial infection, prolonged inflammation, and inferior vessel remodeling. In this work, polypyrrole (Ppy) was added into the hybrid hydrogel containing polyvinyl alcohol (PVA), polyethylene glycol (PEG), and hyaluronan (HA) to acquire superior mechanism and photothermal ability. The Ppy composited hybrid hydrogel could effectively kill bacteria through accumulating heat on the hydrogel surface. RNA-Seq analysis shows that the heat accumulation could enhance phagosome of macrophage and M1 activation, which further accelerate bacteria clearance. Benefitting from the bacteria clearance, macrophage could transform its phenotype to M2 in Ppy composited hybrid hydrogel group with near infrared light (NIR) stimulation. The related genes expression in keratinization, keratinocyte differentiation, and establishment of the skin barrier in the skin were up-regulated and collagen and vascular endothelial growth factor (VEGF) expression level are also enhanced. In summary, Ppy composited hybrid hydrogel could effectively solve the issues of infection and poor wound healing in diabetic foot ulcers, making it an ideal candidate dressing for the treatment of chronic wounds.

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自愈合PY-水凝胶通过近红外引发的增强杀菌和巨噬细胞协调作用促进糖尿病皮肤伤口愈合。

糖尿病足溃疡不愈合是一个棘手的公共卫生问题，其原因是细菌感染未得到控制、炎症持续时间过长以及血管重塑不良。在这项研究中，聚吡咯（Ppy）被添加到含有聚乙烯醇（PVA）、聚乙二醇（PEG）和透明质酸（HA）的混合水凝胶中，以获得优异的机理和光热能力。Ppy 复合杂化水凝胶可通过在水凝胶表面积聚热量来有效杀灭细菌。RNA-Seq分析表明，蓄热可增强巨噬细胞的吞噬功能和M1活化，从而进一步加速细菌的清除。在近红外光（NIR）的刺激下，Ppy 复合杂化水凝胶组中的巨噬细胞可从细菌清除中获益，将其表型转变为 M2。皮肤角质化、角质细胞分化和皮肤屏障建立的相关基因表达上调，胶原蛋白和血管内皮生长因子（VEGF）的表达水平也有所提高。总之，Ppy 复合杂化水凝胶能有效解决糖尿病足溃疡感染和伤口愈合不良的问题，是治疗慢性伤口的理想候选敷料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomaterials 工程技术-材料科学：生物材料

CiteScore

26.00

自引率

2.90%

发文量

565

审稿时长

46 days

期刊介绍： Biomaterials is an international journal covering the science and clinical application of biomaterials. A biomaterial is now defined as a substance that has been engineered to take a form which, alone or as part of a complex system, is used to direct, by control of interactions with components of living systems, the course of any therapeutic or diagnostic procedure. It is the aim of the journal to provide a peer-reviewed forum for the publication of original papers and authoritative review and opinion papers dealing with the most important issues facing the use of biomaterials in clinical practice. The scope of the journal covers the wide range of physical, biological and chemical sciences that underpin the design of biomaterials and the clinical disciplines in which they are used. These sciences include polymer synthesis and characterization, drug and gene vector design, the biology of the host response, immunology and toxicology and self assembly at the nanoscale. Clinical applications include the therapies of medical technology and regenerative medicine in all clinical disciplines, and diagnostic systems that reply on innovative contrast and sensing agents. The journal is relevant to areas such as cancer diagnosis and therapy, implantable devices, drug delivery systems, gene vectors, bionanotechnology and tissue engineering.