A BODIPY-based “turn on” near-infrared fluorescence probe for specific detection of cysteine

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2024-11-13 DOI:10.1016/j.saa.2024.125441

Xiaozong Dou , Weiqi Liu , Yuhui Luo , Li Zhang , Jia Luo , Chenfeng Wu , Tiejun Huang , Xingling Pu

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引用次数: 0

Abstract

This study synthesized a 4,4-difluoro-2,6-di (1-octyl, 5-esteryluridine)-8-(3,4,5-tri (ethanoxy) phenyl) fluoroboron dipyrrole (BODIPY-A) near-infrared fluorescent probe based on 4,4-difluoro-2,6-diiodio-8-(3,4,5-tri (ethanoxy) phenyl) fluoroboron dipyrrole and 1-octanyl, 5-carboxyuracil. The emission wavelength of BODIPY-A probe is 672 nm, located in the near-infrared region, and it possesses benefits such as deep tissue penetration, low background self-fluorescence, and minimal light damage. The BODIPY-A probe exhibits a good turn-on fluorescence response to Cys through nucleophilic substitution reaction with cysteine (Cys), and can eliminate interference from homocysteine (Hcy) and glutathione (GSH). The BODIPY-A probe has been applied to the detection of Cys, with a linear range and detection limit of 0–90 μM and 0.3 μM, respectively. The BODIPY-A probe was applied to analyze the serum samples, achieving an absolute recovery rate ranging from 95 %-99 % and a relative standard deviation (RSD) of 0.031 %-0.371 %. Research has shown that the BODIPY-A probe has the hope to be used for sensitive detection of Cys in the human body.

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基于 BODIPY 的 "开启式 "近红外荧光探针，用于特异性检测半胱氨酸。

本研究合成了一种 4,4-二氟-2,6-二（1-辛基，5-酯基尿苷）-8-（3,4,5-三（乙氧基）苯基）氟硼二吡咯（BODIPY-A）近红外荧光探针，该探针基于 4、BODIPY-A 是一种近红外荧光探针，基于 4,4-二氟-2,6-二碘-8-(3,4,5-三(乙氧基)苯基)氟硼二吡咯和 1-辛酰 5-羧基尿嘧啶。BODIPY-A 探针的发射波长为 672 纳米，位于近红外区域，具有组织穿透深、本底自发荧光低、光损伤小等优点。BODIPY-A 探针通过与半胱氨酸（Cys）发生亲核取代反应，对 Cys 具有良好的开启荧光响应，并能消除同型半胱氨酸（Hcy）和谷胱甘肽（GSH）的干扰。BODIPY-A 探针已应用于 Cys 的检测，其线性范围和检测限分别为 0-90 μM 和 0.3 μM。应用 BODIPY-A 探针分析血清样品，绝对回收率为 95 %-99 %，相对标准偏差（RSD）为 0.031 %-0.371 %。研究表明，BODIPY-A 探针有望用于人体内 Cys 的灵敏检测。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.