A defensive pathway from NAC and TCP transcription factors activates a BAHD acyltransferase for (Z)-3-hexenyl acetate biosynthesis to resist herbivore in tea plant (Camellia sinensis).

IF 9.4 1区 生物学 Q1 Agricultural and Biological Sciences
New Phytologist Pub Date : 2024-11-17 DOI:10.1111/nph.20283
Honglian Gu, Jiaxing Li, Dahe Qiao, Mei Li, Yingjie Yao, Hui Xie, Ke-Lin Huang, Shengrui Liu, De-Yu Xie, Chaoling Wei, Junyan Zhu
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Abstract

Numerous herbivore-induced plant volatiles (HIPVs) play important roles in plant defense. In tea plants (Camellia sinensis), (Z)-3-hexenyl acetate (3-HAC) has been characterized as associated with resistance to herbivores. To date, how tea plants biosynthesize and regulate 3-HAC to resist herbivores remain unclear. Based on transcriptomes assembled from Ectropis obliqua-fed leaves, a cDNA encoding BAHD acyltransferase, namely CsCHAT1, was highly induced in leaves fed with E. obliqua. Enzymatic assays showed that CsCHAT1 converted (Z)-3-hexenol into 3-HAC. Further suppression of CsCHAT1 expression reduced the accumulation of 3-HAC and lowered the resistance of tea plants to E. obliqua, while 3-HAC replenishment rescued the reduced resistance of CsCHAT1-silenced tea plants against E. obliqua. Two transcription factors (TFs), CsNAC30 and CsTCP11, were co-expressed with CsCHAT1. An integrative approach of biochemistry, DNA-protein interaction, gene silencing, and metabolic profiling revealed that the two TFs positively regulated the expression of CsCHAT1. The suppression of either one decreased the production of 3-HAC and eliminated the resistance of tea plants to E. obliqua. Notably, the suppression of either one considerably impaired JA-induced 3-HAC biosynthesis in tea plant. The proposed pathway can be targeted for innovative agro-biotechnologies protecting tea plants from damage by E. obliqua.

来自 NAC 和 TCP 转录因子的防御途径可激活 BAHD 乙酰转移酶,促进 (Z)-3- 己烯基乙酸酯的生物合成,从而抵御茶树(Camellia sinensis)中的草食动物。
许多食草动物诱导的植物挥发物(HIPVs)在植物防御中发挥着重要作用。在茶树(Camellia sinensis)中,(Z)-3-己烯基乙酸酯(3-HAC)被认为与抵抗食草动物有关。迄今为止,茶树如何生物合成和调节 3-HAC 以抵御食草动物仍不清楚。根据从Ectropis obliqua喂食的叶片中收集的转录组,编码BAHD酰基转移酶(即CsCHAT1)的cDNA在Ectropis obliqua喂食的叶片中被高度诱导。酶测定显示,CsCHAT1 能将(Z)-3-己烯醇转化为 3-HAC。进一步抑制 CsCHAT1 的表达可减少 3-HAC 的积累,并降低茶树对 E. obliqua 的抗性,而 3-HAC 的补充则可挽救被 CsCHAT1 沉默的茶树对 E. obliqua 抗性的降低。两个转录因子(TFs)CsNAC30 和 CsTCP11 与 CsCHAT1 共同表达。生物化学、DNA 蛋白相互作用、基因沉默和代谢分析等综合方法显示,这两个转录因子对 CsCHAT1 的表达有正向调节作用。抑制其中任何一个都会减少 3-HAC 的产生,并消除茶树对 E. obliqua 的抗性。值得注意的是,抑制其中任何一种都会大大削弱 JA 诱导的茶树 3-HAC 生物合成。所提出的途径可作为创新农业生物技术的目标,保护茶树免受欧鼠李的损害。
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来源期刊
New Phytologist
New Phytologist PLANT SCIENCES-
CiteScore
17.60
自引率
5.30%
发文量
728
审稿时长
1 months
期刊介绍: New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.
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