Capsular polysaccharide structure of Acinetobacter baumannii K58 from clinical isolate MRSN31468

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2024-11-06 DOI:10.1016/j.carres.2024.109307

Wei Zou , Evguenii Vinogradov , Frank St-Michael , Dean Williams , Lillian Zou , Jenny Peters , Melanie Arbour , Greg Harris , Wangxue Chen , Danielle Peters

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引用次数: 0

Abstract

Capsular polysaccharides (CPS) of Acinetobacter baumannii is a virulence factor with diverse structures. CPS are produced by the CPS biosynthesis gene cluster in their K locus (KL). However, CPS variations may occur due to insertion of additional genes from external sources, e.g., prophages. Recently, the CPS structure from a clinical isolate, BAL062 which includes KL58 locus, was found to have a pseudaminic acid isomer (8ePse5NAc7NAc) as a result of prophage inserted epaA/epaB genes. Here, we report a CPS structure produced by A. baumannii strain MRSN31468 which also belongs to a KL58 type. The K58 CPS structure was determined by 1D and 2D NMR analysis of the oligosaccharides derived from the CPS by a phage depolymerase, and supported by the sugar composition analysis. The K58 CPS structure has the following tetra saccharide repeating unit.

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The K58 CPS differs from the CPS from BAL062 only by replacing 8-epimerized β-8ePse5NAc7NAc with β-Pse5NAc7NAc.

Abstract Image

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从临床分离物 MRSN31468 中提取的鲍曼不动杆菌 K58 的胶囊多糖结构。

鲍曼不动杆菌的胶囊多糖（CPS）是一种具有多种结构的毒力因子。CPS 由其 K 基因座（KL）中的 CPS 生物合成基因簇产生。然而，由于插入了额外的外部基因（如噬菌体），CPS 可能会发生变异。最近，人们发现临床分离株 BAL062 的 CPS 结构（包括 KL58 基因座）中有一个伪氨基酸异构体（8ePse5NAc7NAc），这是噬菌体插入 epaA/epaB 基因的结果。在此，我们报告了鲍曼不动杆菌 MRSN31468 菌株产生的一种 CPS 结构，它也属于 KL58 型。K58 CPS结构是通过对噬菌体解聚酶从CPS中提取的寡糖进行一维和二维核磁共振分析确定的，并得到了糖成分分析的支持。K58 CPS 结构具有以下四糖重复单元。K58 CPS 与 BAL062 的 CPS 的不同之处仅在于用 β-Pse5NAc7NAc 取代了 8-epimerized β-8ePse5NAc7NAc。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".