Role of gut microbiome in developing necrotizing enterocolitis.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Cevher Karaca, Hatice Aysun Mercimek Takcı
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引用次数: 0

Abstract

Necrotizing enterocolitis (NEC) is one of the most devastating intestinal diseases observed in preterm in the first days of life. Researchers have recently focused on potential predictive biomarkers for early and concomitant diagnoses. Thus, we inquired about the linkage of intestinal dysbiosis, one of the most important factors in NEC development to the gut microbiota. In this study, the systematic differences in the bacterial composition between neonates affected by NEC and healthy newborns were highlighted by metagenomic analysis. The next-generation sequencing of the V3-V4 variable region of the 16S rRNA gene and gene-specific qPCR analyzed the untargeted gut microbiota. Total bacteria, total and fecal coliform loads in stool samples with NEC were higher than control. OTU-level relative abundances of NEC infant was characterized by Firmicutes and Bacteroidetes at phylum levels. At the genus level, NEC stool was identified by the lack of Klebsiella and the presence of Roseburia, Blautia, and Parasutterella. Finally, Clostridium fessum was the predominant species of Clostridium genus in disease and healthy specimens at the species level, whereas Clostridium jeddahitimonense was at NEC diagnosis. Despite a strong relationship between pathophysiology and characterization of gut microbiota at a clinical diagnosis of NEC, our results emphasize the broad difficulty in identifying potential biomarkers.

肠道微生物组在发生坏死性小肠结肠炎中的作用。
坏死性小肠结肠炎(NEC)是早产儿在出生后最初几天观察到的最具破坏性的肠道疾病之一。最近,研究人员将重点放在早期诊断和并发症的潜在预测性生物标志物上。因此,我们探究了肠道菌群失调(NEC 发病的最重要因素之一)与肠道微生物群之间的联系。在这项研究中,元基因组分析凸显了受 NEC 影响的新生儿与健康新生儿之间细菌组成的系统性差异。16S rRNA基因V3-V4可变区的下一代测序和基因特异性qPCR分析了非靶向肠道微生物群。NEC粪便样本中的细菌总数、总大肠菌群和粪大肠菌群均高于对照组。在菌门水平上,NEC 婴儿粪便样本中的 OTU 相对丰度以固着菌和类杆菌为特征。在属一级,NEC 婴儿粪便样本中没有克雷伯氏菌,但有罗斯伯氏菌、布劳氏菌和副菌。最后,在种的层面上,疾病和健康标本中的梭状芽孢杆菌属主要是费萨姆梭状芽孢杆菌,而在 NEC 诊断中主要是吉达梭状芽孢杆菌。尽管病理生理学与 NEC 临床诊断时的肠道微生物群特征之间存在密切关系,但我们的研究结果强调了确定潜在生物标志物的巨大困难。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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