Impact of aerobic exercise on brain metabolism: Insights from spatial metabolomic analysis

IF 2.6 3区心理学 Q2 BEHAVIORAL SCIENCES

Behavioural Brain Research Pub Date : 2024-11-15 DOI:10.1016/j.bbr.2024.115339

Jiaping Zheng , Wei Luo , Chenghua Kong , Wenhuo Xie , Xiuyun Chen , Jiaxian Qiu , Kexin Wang , Hong Wei , Yu Zhou

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引用次数: 0

Abstract

Background

Exercise is acknowledged for its beneficial effects on brain health; however, the intricate underlying molecular mechanisms remain poorly understood. Aims: This study aimed to explore aerobic exercise-induced metabolic alterations in the brain. Methods: We conducted an eight-week treadmill running exercise program in two-month-old male C57/BL6J mice. Body weight, serum lipid, glucose levels, and spatial cognition were measured. Spatial metabolomic analysis was performed to compare the metabolomic profiles across different brain regions. Immunohistochemical methods were used to compare the expression of carnitine palmitoyltransferase 1c (CPT1c). Results: Exercise induced significant changes in the analysed metabolomic profiles. There were 904 differentially expressed metabolites (DEMs) detected in the whole brain section. Notable alterations in lipid profiles were observed, and among the 292 lipids detected, there were 74 (25.34 %), 85 (29.11 %), and 78 (26.71 %) lipids differentially expressed in the hippocampus, thalamus, and hypothalamus of the Exe group, respectively. Lipid metabolism related pathways and enzymes were also altered, with L-carnitine and CPT1c upregulated in the three regions (p<0.05), and epinephrine levels decreased in the hippocampus (p<0.05). Furthermore, the vitamin B6 metabolism pathway was altered in the hypothalamus.

Conclusions

This study highlighted the significant changes in lipid metabolism induced by involuntary exercise in the brains of young male mice. Exercise also altered epinephrine levels and the vitamin B12 metabolic pathway in specific brain regions, which indicated the multifaceted effects of exercise on the brain.

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有氧运动对大脑新陈代谢的影响：空间代谢组分析的启示。

背景：目的：本研究旨在探讨有氧运动诱导的脑代谢改变：方法：我们对两个月大的雄性 C57/BL6J 小鼠进行了为期八周的跑步机跑步锻炼。方法：我们对两个月大的雄性C57/BL6J小鼠进行了为期八周的跑步训练，测量了体重、血脂、血糖水平和空间认知能力。进行了空间代谢组学分析，以比较不同脑区的代谢组学特征。免疫组化方法用于比较肉碱棕榈酰基转移酶 1c（CPT1c）的表达：结果：运动导致所分析的代谢组谱发生了显著变化。在全脑切片中检测到 904 种差异表达代谢物（DEMs）。在检测到的 292 种脂质中，有 74 种（25.34%）、85 种（29.11%）和 78 种（26.71%）脂质在 Exe 组的海马、丘脑和下丘脑中有差异表达。脂质代谢相关通路和酶也发生了改变，左旋肉碱和 CPT1c 在这三个区域中上调（pConclusions：本研究强调了非自主运动在年轻雄性小鼠大脑中引起的脂质代谢的显著变化。运动还改变了特定脑区的肾上腺素水平和维生素 B12 代谢途径，这表明运动对大脑的影响是多方面的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Behavioural Brain Research 医学-行为科学

CiteScore

5.60

自引率

0.00%

发文量

383

审稿时长

61 days

期刊介绍： Behavioural Brain Research is an international, interdisciplinary journal dedicated to the publication of articles in the field of behavioural neuroscience, broadly defined. Contributions from the entire range of disciplines that comprise the neurosciences, behavioural sciences or cognitive sciences are appropriate, as long as the goal is to delineate the neural mechanisms underlying behaviour. Thus, studies may range from neurophysiological, neuroanatomical, neurochemical or neuropharmacological analysis of brain-behaviour relations, including the use of molecular genetic or behavioural genetic approaches, to studies that involve the use of brain imaging techniques, to neuroethological studies. Reports of original research, of major methodological advances, or of novel conceptual approaches are all encouraged. The journal will also consider critical reviews on selected topics.