Characterizing pregnancy losses in lactating Holstein cows receiving a fixed-timed artificial insemination protocol

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2024-11-13 DOI:10.1016/j.anireprosci.2024.107644

A.K. Munhoz , R.F. Cooke , C.P. Prado , S.K. Munhoz , M.C.G. de Sousa , V.M.P. da Silva , K.G. Pohler , B.I. Cappellozza , J.L.M. Vasconcelos

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Abstract

This experiment assessed pregnancy losses from day 31 of gestation to calving in lactating Holstein cows reared in tropical conditions, and evaluated if serum concentrations of haptoglobin and pregnancy-associated glycoproteins (PAGs) during early gestation differs according to pregnancy losses. Cows (708 primiparous and 844 multiparous) were assigned to an ovulation synchronization + fixed-time artificial insemination (FTAI) protocol (day −11–0 of the experiment). Pregnancy status was verified using transrectal ultrasonography on days 31, 62, 120, and according to calf birth. Blood samples were collected from all cows on day 24, and from cows diagnosed as pregnant on day 31. Pregnancy losses were greater (P < 0.01) from day 31–62 (12.8 %) and day 120 to calving (12.1 %) compared with day 62–120 (6.42 %). Pregnancy losses were greater in multiparous compared with primiparous cows from day 31–62 (17.1 vs. 9.5 %) and from 120 to calving (15.4 vs. 7.7 %). Serum PAGs concentrations on day 31 were less (P ≤ 0.03) in cows that lost the pregnancy from day 31–62 (3.57 ng/mL) and from day 62–120 (4.40 ng/mL) compared to cows that maintained the pregnancy (5.57 and 5.66 ng/mL, respectively). Cows that experienced pregnancy loss from day 31–62 had greater (P = 0.05) serum haptoglobin concentrations on day 24 (0.414 mg/mL) compared with cows that maintained the pregnancy (0.271 mg/mL). Collectively, this experiment provides novel information about pregnancy losses after day 31 of gestation in lactating Holstein cows reared in tropical environments.

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接受固定时间人工授精方案的荷斯坦泌乳奶牛的妊娠损失特征。

本实验评估了在热带条件下饲养的泌乳荷斯坦奶牛从妊娠第31天到产犊期间的妊娠损失，并评估了妊娠早期血清中的血红蛋白和妊娠相关糖蛋白（PAGs）浓度是否因妊娠损失而有所不同。708头初产母牛和844头多产母牛被分配到排卵同步化+固定时间人工授精（FTAI）方案中（实验的第-11-0天）。第 31 天、第 62 天、第 120 天以及犊牛出生时，使用经直肠超声波检查验证妊娠状态。第 24 天采集所有奶牛的血样，第 31 天采集确诊妊娠奶牛的血样。与第 62-120 天（6.42%）相比，第 31-62 天（12.8%）和第 120 天至产犊（12.1%）的妊娠损失更大（P < 0.01）。与初产母牛相比，多胎母牛在第31-62天（17.1%对9.5%）和第120天到产犊期间（15.4%对7.7%）的妊娠损失更大。与维持妊娠的奶牛（分别为5.57和5.66纳克/毫升）相比，在第31-62天（3.57纳克/毫升）和第62-120天（4.40纳克/毫升）失去妊娠的奶牛在第31天的血清PAGs浓度较低（P≤0.03）。与维持妊娠的奶牛（0.271 毫克/毫升）相比，第 31-62 天发生妊娠损失的奶牛在第 24 天的血清隐血红蛋白浓度更高（P = 0.05）（0.414 毫克/毫升）。总之，本实验为热带环境下饲养的荷斯坦泌乳奶牛在妊娠第31天后的妊娠损失提供了新的信息。

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来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.