Metagenomic analysis of a top-down enriched straw biodegradation synthetic bacterial consortium (StrBsyn) facilitates anaerobic rice straw degradation

IF 6.9 2区 环境科学与生态学 Q1 ENGINEERING, CHEMICAL
Yanxu Ma , Haixia Pan , Zhongqing Feng , Pengju Li , Mengyue Fu , Xianliang Yi , Yang Liu , Xiaojing Yang , Xu Zhao , Jingjing Zhan , Zhiqiang Zhao , Hao Zhou
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Abstract

Lignocellulose is a complex and recalcitrant component of plant biomass. Traditional sources of lignocellulose-degrading microorganisms are the rumen system of herbivores and soil microorganisms, with limited studies on marine sediments. In this study, we employed a "top-down" approach to construct a straw biodegradation synthetic bacterial consortium (StrBsyn) capable of degrading lignocellulose from offshore marine sediments. It achieved 66.74 % cellulose, 63.79 % hemicellulose, and 42.85 % lignin conversion within 30 days using 0.2 % rice straws. Additionally, the maximum concentrations of total organic carbon (TOC) and total volatile fatty acids (TVFA) reached 4.66 g/ml and 15.10 g/L, respectively, with a significant increase in pH. β-glucosidase, xylanase, laccase, and lignin peroxidase activities were detected in StrBsyn. Metagenomic sequencing revealed a dynamic shift in the community composition and functional genes. The taxonomic assignment of these functional genes suggested the genera Clostridium, Glomeromyces, and Sedimentobacter as major contributors to carbohydrate transport, metabolism, and lignin degradation processes. This study demonstrated the significant potential of harnessing the power of marine sediment microbial communities for sustainable and efficient anaerobic lignocellulose bioconversion.
自上而下富集秸秆生物降解合成细菌联合体(StrBsyn)的元基因组分析促进厌氧水稻秸秆降解
木质纤维素是植物生物质中一种复杂的难降解成分。木质纤维素降解微生物的传统来源是食草动物的瘤胃系统和土壤微生物,对海洋沉积物的研究有限。在本研究中,我们采用 "自上而下 "的方法构建了一个秸秆生物降解合成细菌联合体(StrBsyn),该联合体能够降解近海海洋沉积物中的木质纤维素。在使用 0.2 % 稻草的 30 天内,它实现了 66.74 % 的纤维素、63.79 % 的半纤维素和 42.85 % 的木质素转化。此外,总有机碳(TOC)和总挥发性脂肪酸(TVFA)的最高浓度分别达到 4.66 克/毫升和 15.10 克/升,pH 值也显著增加。元基因组测序揭示了群落组成和功能基因的动态变化。这些功能基因的分类归属表明,梭状芽孢杆菌属、革兰霉属和沉积细菌属是碳水化合物运输、代谢和木质素降解过程的主要贡献者。这项研究表明,利用海洋沉积物微生物群落的力量进行可持续、高效的厌氧木质纤维素生物转化具有巨大潜力。
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来源期刊
Process Safety and Environmental Protection
Process Safety and Environmental Protection 环境科学-工程:化工
CiteScore
11.40
自引率
15.40%
发文量
929
审稿时长
8.0 months
期刊介绍: The Process Safety and Environmental Protection (PSEP) journal is a leading international publication that focuses on the publication of high-quality, original research papers in the field of engineering, specifically those related to the safety of industrial processes and environmental protection. The journal encourages submissions that present new developments in safety and environmental aspects, particularly those that show how research findings can be applied in process engineering design and practice. PSEP is particularly interested in research that brings fresh perspectives to established engineering principles, identifies unsolved problems, or suggests directions for future research. The journal also values contributions that push the boundaries of traditional engineering and welcomes multidisciplinary papers. PSEP's articles are abstracted and indexed by a range of databases and services, which helps to ensure that the journal's research is accessible and recognized in the academic and professional communities. These databases include ANTE, Chemical Abstracts, Chemical Hazards in Industry, Current Contents, Elsevier Engineering Information database, Pascal Francis, Web of Science, Scopus, Engineering Information Database EnCompass LIT (Elsevier), and INSPEC. This wide coverage facilitates the dissemination of the journal's content to a global audience interested in process safety and environmental engineering.
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