Analysis on characteristics and multilocus sequence typing of Clostridium perfringens in western China.

IF 3.9 2区医学 Q1 INFECTIOUS DISEASES

Journal of Antimicrobial Chemotherapy Pub Date : 2024-11-15 DOI:10.1093/jac/dkae399

Shao Yanxia, Wang Xuewei, Li Gang, Jia Wei

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引用次数: 0

Abstract

Objectives: The objective of this study was to identify and analyse the distribution characteristics, toxin genotyping and antimicrobial susceptibility of Clostridium perfringens and to investigate its resistance mechanisms and genetic characteristics.

Methods: The MICs of various antibiotics against C. perfringens were determined using the agar dilution method, and resistance genes and toxin genotypes were detected by PCR. Genetic relationships were analysed using MLST. WGS was conducted on the DNB system and PacBio platforms.

Results: Analysis of 36 strains of C. perfringens revealed that the major toxin types were types C and F, with 86.1% of the strains isolated from bile samples. Of these, 30.6% of the strains exhibited MDR, with resistance rates of 75.0%, 52.8% and 52.8% for penicillin, clindamycin and ampicillin, respectively; however, no resistance to metronidazole and carbapenems was observed. MLST analysis identified 29 STs, including 14 novel types. ST221 and ST498 were the dominant types. The WGS revealed that the most prevalent virulence factors are plc (100.0%), nagH (100.0%), colA (100.0%), nanJ (100.0%), entB (100%), nanH (97.0%), entA (97.0%) and nanI (90.9%). Among these factors, the primary determinants of tetracycline resistance are tetA (66.7%) and tetB (78.8%), which represent the most frequently detected antibiotic resistance genes.

Conclusions: This study indicates that the infection rate of C. perfringens is relatively high, with the majority of isolated strains exhibiting MDR. The observed high levels of antibiotic resistance, combined with the significant genetic diversity of these strains, suggest a potential public health risk.

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中国西部产气荚膜梭菌特征及多焦点序列分型分析

研究目的本研究旨在确定和分析产气荚膜梭菌的分布特征、毒素基因分型和抗菌药敏感性，并研究其耐药机制和遗传特征：方法：采用琼脂稀释法测定各种抗生素对产气荚膜梭菌的 MIC，并通过 PCR 检测耐药基因和毒素基因型。利用 MLST 分析了遗传关系。在 DNB 系统和 PacBio 平台上进行了 WGS 分析：对 36 株产气荚膜杆菌的分析表明，主要毒素类型为 C 型和 F 型，86.1% 的菌株从胆汁样本中分离出来。其中，30.6%的菌株表现出MDR，对青霉素、克林霉素和氨苄西林的耐药率分别为75.0%、52.8%和52.8%；但未观察到对甲硝唑和碳青霉烯类的耐药。MLST 分析确定了 29 种 ST，包括 14 种新型。ST221 和 ST498 是主要类型。WGS显示，最普遍的毒力因子是plc（100.0%）、nagH（100.0%）、colA（100.0%）、nanJ（100.0%）、entB（100%）、nanH（97.0%）、entA（97.0%）和nanI（90.9%）。在这些因素中，四环素耐药性的主要决定因素是 tetA（66.7%）和 tetB（78.8%），它们代表了最常检测到的抗生素耐药基因：本研究表明，产气荚膜杆菌的感染率相对较高，大多数分离菌株表现出 MDR。观察到的高水平抗生素耐药性与这些菌株的显著遗传多样性相结合，表明存在潜在的公共卫生风险。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Antimicrobial Chemotherapy 医学-传染病学

CiteScore

9.20

自引率

5.80%

发文量

423

审稿时长

2-4 weeks

期刊介绍： The Journal publishes articles that further knowledge and advance the science and application of antimicrobial chemotherapy with antibiotics and antifungal, antiviral and antiprotozoal agents. The Journal publishes primarily in human medicine, and articles in veterinary medicine likely to have an impact on global health.