Insight into metabolomics analysis of large yellow croaker liver (Larimichthys crocea) during packaging bag transport

IF 2.2 3区农林科学 Q2 FISHERIES

Aquaculture International Pub Date : 2024-11-14 DOI:10.1007/s10499-024-01766-z

Haofeng Sun, Fukun Gui, Dejun Feng, Ping Wang, Xiaoyu Qu, Shuai Niu, Guangyang Zhang

{"title":"Insight into metabolomics analysis of large yellow croaker liver (Larimichthys crocea) during packaging bag transport","authors":"Haofeng Sun, Fukun Gui, Dejun Feng, Ping Wang, Xiaoyu Qu, Shuai Niu, Guangyang Zhang","doi":"10.1007/s10499-024-01766-z","DOIUrl":null,"url":null,"abstract":"<div><p>The liver metabolomics and related biochemical indices of large yellow croaker were investigated during packaging and oxygenation transport. The large yellow croaker was packed into bags and transported at a constant temperature of 20 ℃. Serum and liver samples were collected after 0, 6, 12, 18, and 24 h of transportation for subsequent analysis. The results showed that transport time had a significant effect on serum creatinine (CR) and glutamine transaminase (AST) levels, suggesting that the kidney and liver tissues of large yellow croaker were affected. Transport stress impacted the antioxidant capacity of large yellow croaker liver, indicated by increased activities of catalase (CAT), malondialdehyde (MDA), and alkaline phosphatase (AKP), which altered immune capacity. In the liver metabolome, we identified five metabolic pathways that were enriched based on differential metabolites and compared across different transport times. These pathways include biosynthesis, lipid, cofactor and vitamin, amino acid, and carbohydrate metabolism. These pathways may impact the morphology of liver cells, as well as the antioxidant and immune abilities of large yellow croaker. Additionally, the statistical analysis of the metabolites at each transportation time revealed that histidylarginine and 3-(4-hydroxyphenyl) pyruvate were consistently downregulated which serve as signature metabolites during live large yellow croaker transportation.</p></div>","PeriodicalId":8122,"journal":{"name":"Aquaculture International","volume":"33 1","pages":""},"PeriodicalIF":2.2000,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture International","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s10499-024-01766-z","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

The liver metabolomics and related biochemical indices of large yellow croaker were investigated during packaging and oxygenation transport. The large yellow croaker was packed into bags and transported at a constant temperature of 20 ℃. Serum and liver samples were collected after 0, 6, 12, 18, and 24 h of transportation for subsequent analysis. The results showed that transport time had a significant effect on serum creatinine (CR) and glutamine transaminase (AST) levels, suggesting that the kidney and liver tissues of large yellow croaker were affected. Transport stress impacted the antioxidant capacity of large yellow croaker liver, indicated by increased activities of catalase (CAT), malondialdehyde (MDA), and alkaline phosphatase (AKP), which altered immune capacity. In the liver metabolome, we identified five metabolic pathways that were enriched based on differential metabolites and compared across different transport times. These pathways include biosynthesis, lipid, cofactor and vitamin, amino acid, and carbohydrate metabolism. These pathways may impact the morphology of liver cells, as well as the antioxidant and immune abilities of large yellow croaker. Additionally, the statistical analysis of the metabolites at each transportation time revealed that histidylarginine and 3-(4-hydroxyphenyl) pyruvate were consistently downregulated which serve as signature metabolites during live large yellow croaker transportation.

查看原文本刊更多论文

大黄鱼肝脏（Larimichthys crocea）在包装袋运输过程中的代谢组学分析透视

研究了大黄鱼在包装和充氧运输过程中的肝脏代谢组学和相关生化指标。将大黄鱼装入袋中，在 20 ℃ 恒温条件下运输。分别在运输的 0、6、12、18 和 24 小时后采集血清和肝脏样本进行后续分析。结果表明，运输时间对血清肌酐（CR）和谷丙转氨酶（AST）水平有显著影响，表明大黄鱼的肾脏和肝脏组织受到了影响。运输应激影响了大黄鱼肝脏的抗氧化能力，表现为过氧化氢酶（CAT）、丙二醛（MDA）和碱性磷酸酶（AKP）活性的增加，从而改变了免疫能力。在肝脏代谢组中，我们根据不同的代谢物确定了五个代谢途径，并在不同的运输时间进行了比较。这些途径包括生物合成、脂质、辅助因子和维生素、氨基酸以及碳水化合物代谢。这些途径可能会影响肝细胞的形态以及大黄鱼的抗氧化和免疫能力。此外，对各运输时间代谢物的统计分析显示，组氨酰精氨酸和 3-（4-羟基苯基）丙酮酸持续下调，这两种代谢物是大黄鱼活体运输过程中的标志性代谢物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Aquaculture International 农林科学-渔业

CiteScore

5.10

自引率

6.90%

发文量

204

审稿时长

1.0 months

期刊介绍： Aquaculture International is an international journal publishing original research papers, short communications, technical notes and review papers on all aspects of aquaculture. The Journal covers topics such as the biology, physiology, pathology and genetics of cultured fish, crustaceans, molluscs and plants, especially new species; water quality of supply systems, fluctuations in water quality within farms and the environmental impacts of aquacultural operations; nutrition, feeding and stocking practices, especially as they affect the health and growth rates of cultured species; sustainable production techniques; bioengineering studies on the design and management of offshore and land-based systems; the improvement of quality and marketing of farmed products; sociological and societal impacts of aquaculture, and more. This is the official Journal of the European Aquaculture Society.