Response Surface Optimization of Solvent-Tolerant Cold-Active Lipase Production by Pseudomonas sp. VITCLP4

IF 2.3 4区化学 Q3 CHEMISTRY, PHYSICAL

Catalysis Letters Pub Date : 2024-11-14 DOI:10.1007/s10562-024-04848-y

V. Iswareya Lakshimi, M. Kavitha

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Abstract

The yield of solvent-tolerant cold-active lipase from halo-tolerant Pseudomonas sp. VITCLP4 was improved by applying statistical methods to precisely combine medium components and other conditions. A two-level Plackett–Burman (PB) design was utilized to screen the most significant variables from eleven variables that influence lipase production. Analysis of variance revealed that Tween-80, peptone, and KH₂PO₄ were statistically significant. Optimum levels of selected variables were ascertained by one-factor-at-a-time (OFAT) analysis. The selected three components' optimum values and interactive effects were established by response surface methodology (RSM) using a five-level-three-factor approach based on the central composite design (CCD). The optimized medium containing Tween-80, 0.6% (v/v), peptone, 0.85% (w/v), KH₂PO₄, 0.1% (w/v), yeast extract, 0.1% (w/v), olive oil, 0.2% (v/v) and inoculum size, 0.2% (v/v) resulted in maximum lipase production of 1356 Units mL⁻¹ min⁻¹ with 7.9-fold increase in the yield. This study provides insights into enhancing lipase production statistically with optimized resources that can be utilized in bioprocess studies and industrial applications.

Graphical Abstract

Abstract Image

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VITCLP4 假单胞菌生产耐溶剂冷活性脂肪酶的响应面优化

通过应用统计学方法精确组合培养基成分和其他条件，提高了耐晕假单胞菌VITCLP4的耐溶剂冷活性脂肪酶产量。该研究采用了两级普拉克特-伯曼（PB）设计，从 11 个影响脂肪酶产量的变量中筛选出最重要的变量。方差分析显示，吐温-80、蛋白胨和 KH2PO4 具有显著的统计学意义。所选变量的最佳水平是通过一次一因素（OFAT）分析确定的。采用基于中央复合设计（CCD）的五级三因素法，通过响应面方法（RSM）确定了所选三个组分的最佳值和交互效应。含有吐温-80（0.6% (v/v)）、蛋白胨（0.85% (w/v)）、KH2PO4（0.1% (w/v)）、酵母提取物（0.1% (w/v)）、橄榄油（0.2% (v/v)）和接种体大小（0.2% (v/v)）的优化培养基可使脂肪酶产量达到 1356 单位 mL-1 min-1，产量增加了 7.9 倍。这项研究为利用优化资源提高脂肪酶产量提供了见解，这些资源可用于生物工艺研究和工业应用。图文摘要

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来源期刊

Catalysis Letters 化学-物理化学

CiteScore

5.70

自引率

3.60%

发文量

327

审稿时长

1 months

期刊介绍： Catalysis Letters aim is the rapid publication of outstanding and high-impact original research articles in catalysis. The scope of the journal covers a broad range of topics in all fields of both applied and theoretical catalysis, including heterogeneous, homogeneous and biocatalysis. The high-quality original research articles published in Catalysis Letters are subject to rigorous peer review. Accepted papers are published online first and subsequently in print issues. All contributions must include a graphical abstract. Manuscripts should be written in English and the responsibility lies with the authors to ensure that they are grammatically and linguistically correct. Authors for whom English is not the working language are encouraged to consider using a professional language-editing service before submitting their manuscripts.