Zingerone effects on arsenic-induced glucose intolerance and hepatotoxicity in mice via suppression of oxidative stress-mediated hepatic inflammation and apoptosis

IF 3.6 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Trace Elements in Medicine and Biology Pub Date : 2024-11-09 DOI:10.1016/j.jtemb.2024.127562

Mobina Hafezizadeh , Maryam Salehcheh , Shokooh Mohtadi , Esrafil Mansouri , Mohammad Javad Khodayar

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引用次数: 0

Abstract

Background

Arsenic (As), a poisonous metalloid, is widely distributed in air, water, and soil and has been associated with the occurrence of diabetes and liver toxicity. Zingerone (ZNG), one of the active compounds in ginger, has several pharmacological benefits such as antioxidant and anti-inflammatory characteristics. The objective of this research was to assess the protective role of ZNG against arsenic (As)-induced glucose intolerance (GI) and hepatotoxicity in mice.

Methods

Male NMRI mice were treated with ZNG (25, 50, and 100 mg/kg, oral gavage for 29 days) before As administration (10 mg/kg, oral gavage for 29 days). On the 29th day, fasting blood glucose (FBG) and glucose tolerance test were measured. The animals were euthanized (day 30), and samples from blood and tissue (liver and pancreas) were gathered for further evaluations.

Results

Administration of ZNG inhibited As-induced elevation of FBG and GI. Moreover, hepatic tissue damage and decreased Langerhans islets' diameter caused by As administration were improved by ZNG treatment. Pretreatment with ZNG attenuated the elevation of serum liver enzymes induced by As (alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase). Also, the reduction in total thiol content, as well as the decline in antioxidant enzyme activities (catalase, superoxide dismutase, and glutathione peroxidase) and the increase in lipid peroxidation marker (thiobarbituric acid reactive substances) in the liver tissue of As-exposed mice were reversed in ZNG-treated mice. Furthermore, ZNG prevented the increase of hepatic inflammatory markers (nitric oxide and tumor necrosis factor-alpha levels, and protein expression of nuclear factor-kappa B) and apoptosis-related marker (caspase-3 protein expression) in As-treated mice.

Conclusions

This study has provided evidence indicating that ZNG can act as a beneficial agent in preventing As-induced hepatotoxicity and diabetes.

查看原文本刊更多论文

姜酮通过抑制氧化应激介导的肝脏炎症和细胞凋亡，对砷诱导的小鼠葡萄糖耐受不良和肝脏毒性产生影响。

背景：砷（As）是一种有毒的类金属，广泛分布于空气、水和土壤中，与糖尿病和肝中毒的发生有关。姜酮（ZNG）是生姜中的活性化合物之一，具有抗氧化和抗炎等多种药理作用。本研究的目的是评估姜酮对砷（As）诱导的小鼠葡萄糖不耐受（GI）和肝毒性的保护作用：方法：雄性 NMRI 小鼠在服用砷（10 毫克/千克，口服，29 天）之前接受 ZNG 治疗（25、50 和 100 毫克/千克，口服，29 天）。第 29 天，测量空腹血糖 (FBG) 和葡萄糖耐量试验。第 30 天，对动物实施安乐死，并收集血液和组织（肝脏和胰腺）样本以进行进一步评估：结果：服用 ZNG 可抑制 As 引起的 FBG 和 GI 升高。此外，ZNG 还能改善因服用 As 而导致的肝组织损伤和朗格汉斯胰岛直径缩小。ZNG 的预处理减轻了 As 引起的血清肝酶（丙氨酸氨基转移酶、天门冬氨酸氨基转移酶和碱性磷酸酶）的升高。ZNG 还能逆转砷暴露小鼠肝组织中总硫醇含量的减少、抗氧化酶活性（过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶）的下降以及脂质过氧化标记物（硫代巴比妥酸活性物质）的增加。此外，ZNG 还能阻止 As 处理小鼠肝脏炎症标志物（一氧化氮和肿瘤坏死因子-α 水平以及核因子-kappa B 蛋白表达）和细胞凋亡相关标志物（caspase-3 蛋白表达）的增加：本研究提供的证据表明，ZNG 可作为一种有益的药物，预防砷诱导的肝毒性和糖尿病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Trace Elements in Medicine and Biology 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.90%

发文量

202

审稿时长

85 days

期刊介绍： The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.