The impact of antioxidants on antioxidant capacity, DNA fragmentation, and chromatin quality in subfertile men: a randomized clinical trial study.

Revista da Associacao Medica Brasileira (1992) Pub Date : 2024-11-11 eCollection Date: 2024-01-01 DOI:10.1590/1806-9282.20240211

Mesut Sengul, Neslihan Hekim, Ramazan Asci, Sezgin Gunes

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Abstract

Objective: This randomized clinical trial study aims to investigate the effects of antioxidant food supplementation on the total antioxidant capacity of seminal plasma, sperm DNA fragmentation, sperm chromatin quality, and semen parameters.

Methods: In this study, a total of 48 subfertile men with moderate physical activity were included. Group 1 was recommended to use the antioxidant supplements, while antioxidant food supplements were not given to Group 2. Total antioxidant capacity, sperm DNA fragmentation, sperm chromatin structure, hormone levels, physical activities, and semen parameters were evaluated before and after treatment. Total antioxidant capacity, sperm DNA fragmentation, and sperm chromatin structure were assessed using ELISA, transferase dUTP nick end labeling, and aniline blue staining, respectively.

Results: Sperm DNA fragmentation (p=0.003) and histone/protamine ratio (p<0.001) were significantly decreased in the patients receiving antioxidant treatment. There was no statistical difference in the total antioxidant capacity values of the post-treatment groups.

Conclusion: Antioxidant therapy seems to improve sperm DNA fragmentation and histone/protamine ratios in subfertile patients.

Clinical trial registration number: NCT06042738.

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抗氧化剂对亚健康男性抗氧化能力、DNA碎片和染色质质量的影响：一项随机临床试验研究。

研究目的这项随机临床试验研究旨在探讨补充抗氧化食品对精浆总抗氧化能力、精子DNA碎片、精子染色质质量和精液参数的影响：本研究共纳入了 48 名中等体力活动的亚育男性。对治疗前后的总抗氧化能力、精子 DNA 片段、精子染色质结构、激素水平、体力活动和精液参数进行评估。总抗氧化能力、精子DNA片段和精子染色质结构分别采用ELISA、转移酶dUTP缺口标记和苯胺蓝染色法进行评估：结果：精子DNA碎片（p=0.003）和组蛋白/丙胺比率（p结论：抗氧化疗法似乎能改善精子的染色质结构：临床试验注册号：NCT06042738：临床试验注册号：NCT06042738。

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Revista da Associacao Medica Brasileira (1992)

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