Further exploitation of metabolic potential for catechol biodegradation of Klebsiella sp. CD33

Abstract

Microbial-mediated degradation of phenolic pollutants (e.g., catechol) has been a critical concern for sewage treatment, while exploiting the strain resources and fully characterizing the metabolic potential of functional microbes for toxic refractory catechol are the key and study-worthy issues. In this study, up to 32 strains originally isolated from phenol-contaminated environments were phylogenetically affiliated with the genus Klebsiella and identified to have the ability of catechol degradation, with strain CD33 as the excellent one. Single-factor experiments determined that strain CD33 exhibited a highly efficient catechol degradation under the conditions of temperature 35 °C, initial pH value of 7.0, and inoculum volume of 30.0% (v/v). To preliminarily validate the possible pathway of catechol biodegradation, concentration variation of the initial enzyme (i.e., catechol 1,2 dioxygenase) and the corresponding metabolic intermediate (i.e., cis,cis-muconic acid) were detected, suggesting that strain CD33 can degrade the catechol uniquely via the ortho-cleavage pathway. Furthermore, a combination of genome-wide identification, homologous modeling, and gene expression analysis was employed to elucidate the complete pathway of catechol degradation, especially in which a novel branch mediated by CMBL gene was responsible for the direct conversion of (+)-muconolactone into 3-oxoadipic acid. Collectively, this study extends our understanding of catechol degradation of Klebsiella spp., which may provide an alternative promising avenue for the practical application of pollutant remediation.