Comparisons of different electrical stimulation modalities for treating visceral pain in a rodent model of irritable bowel syndrome.

Md Jahangir Alam, Tingting Zhao, John W Wiley, Jiande D Z Chen
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Abstract

The purpose of this study was to investigate the effects of different electrical stimulation methods (bilateral electroacupuncture (BEA), unilateral EA (UEA), transcutaneous electrical acustimulation (TEA, stimulation via surface electrodes placed at acupoints), and sacral nerve stimulation (SNS)) on visceral pain in a rodent model of irritable bowel syndrome (IBS). Ten-day-old male and female pups were treated with 0.2 ml of 0.5% acetic acid (AA) solution. Visceral sensitivity was assessed using an electromyogram (EMG) in response to graded colorectal distension. In the first experiment, bilateral EA at ST36 acupoint was performed with different parameters in male rats to determine the best stimulation parameters. In the second experiment, male rats were randomly assigned into the Sham, BEA, UEA, TEA, and SNS groups to determine the best stimulation method. Lastly, the AA-treated female rats were randomly assigned into the BEA and sham groups to investigate a potential treatment difference between the sexes. Two distinct sets of stimulation parameters were used: Set 1 (100 Hz, 0.5 ms pulse width, 0.1 s ON, 0.4 s OFF, 0.4-3.0 mA current) and Set 2 (25 Hz, 0.5 ms pulse width, 2 s ON, 3 s OFF, 0.4-3.0 mA current).Results (1) The parameter set of 100Hz was found to be most effective in reducing visceral pain. (2) Both acute UEA and TEA effectively relieved visceral pain, whereas acute SNS did not exhibit such an effect. (3) Acute BEA improved visceral pain in both male and female rats.Conclusions These findings suggest that transcutaneous ST36 stimulation is as effective as direct ST36 stimulation and unilateral ST36 stimulation is comparable to bilateral stimulation. Development of a novel therapy using unilateral transcutaneous ST36 stimulation is warranted.

比较不同的电刺激模式在肠易激综合征啮齿动物模型中治疗内脏疼痛的效果。
本研究旨在探讨不同电刺激方法(双侧电针(BEA)、单侧电针(UEA)、经皮电刺激(TEA,通过放置在穴位上的表面电极进行刺激)和骶神经刺激(SNS))对肠易激综合征(IBS)啮齿动物模型内脏疼痛的影响。用 0.5%醋酸(AA)溶液 0.2 毫升处理 10 天大的雌雄幼鼠。使用肌电图(EMG)评估内脏对分级结肠直肠胀气的敏感性。在第一项实验中,雄性大鼠在 ST36 穴位进行了不同参数的双侧 EA,以确定最佳刺激参数。在第二个实验中,雄性大鼠被随机分配到 Sham 组、BEA 组、UEA 组、TEA 组和 SNS 组,以确定最佳刺激方法。最后,经 AA 处理的雌性大鼠被随机分配到 BEA 组和假组中,以研究两性之间潜在的治疗差异。使用了两组不同的刺激参数:结果 (1) 100Hz 的参数集对减轻内脏疼痛最有效。(2)急性 UEA 和 TEA 均能有效缓解内脏疼痛,而急性 SNS 则没有这种效果。(结论 这些研究结果表明,经皮 ST36 刺激与直接 ST36 刺激同样有效,单侧 ST36 刺激与双侧刺激效果相当。有必要开发一种使用单侧经皮 ST36 刺激的新型疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.90
自引率
0.00%
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审稿时长
8 weeks
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