{"title":"Labeling and sorting of avian primordial germ cells utilizing Lycopersicon Esculentum lectin.","authors":"Hiroko Iikawa, Aika Nishina, Mizuki Morita, Yuji Atsuta, Yoshiki Hayashi, Daisuke Saito","doi":"10.1111/dgd.12948","DOIUrl":null,"url":null,"abstract":"<p><p>Avian species are essential resources for human society, with their preservation and utilization heavily dependent on primordial germ cells (PGCs). However, efficient methods for isolating live PGCs from embryos remain elusive in avian species beyond chickens, and even in chickens, existing techniques have shown limited efficiency. In this study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, using Lycopersicon Esculentum (Tomato) lectin (LEL). Notably, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative and migratory potential of chicken PGCs. This approach is anticipated to significantly contribute to the conservation, research, and agricultural industries related to avian species globally.</p>","PeriodicalId":50589,"journal":{"name":"Development Growth & Differentiation","volume":" ","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Development Growth & Differentiation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/dgd.12948","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Avian species are essential resources for human society, with their preservation and utilization heavily dependent on primordial germ cells (PGCs). However, efficient methods for isolating live PGCs from embryos remain elusive in avian species beyond chickens, and even in chickens, existing techniques have shown limited efficiency. In this study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, using Lycopersicon Esculentum (Tomato) lectin (LEL). Notably, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative and migratory potential of chicken PGCs. This approach is anticipated to significantly contribute to the conservation, research, and agricultural industries related to avian species globally.
禽类是人类社会的重要资源，其保存和利用在很大程度上依赖于原始生殖细胞（PGCs）。然而，在鸡以外的禽类物种中，从胚胎中分离活的 PGCs 的有效方法仍然难以找到，即使在鸡中，现有技术也显示出有限的效率。在本研究中，我们提出了一种快速、简单且经济有效的方法，利用番茄凝集素（LEL）对不同禽类物种（包括鲤科和鼠科）的循环期 PGCs 进行标记和分拣。值得注意的是，这种方法既能识别多种 PGC 亚型，又能保留鸡 PGC 的增殖和迁移潜能，因此具有很高的分拣效率。预计这种方法将为全球禽类物种的保护、研究和农业产业做出重大贡献。
期刊介绍：
Development Growth & Differentiation (DGD) publishes three types of articles: original, resource, and review papers.
Original papers are on any subjects having a context in development, growth, and differentiation processes in animals, plants, and microorganisms, dealing with molecular, genetic, cellular and organismal phenomena including metamorphosis and regeneration, while using experimental, theoretical, and bioinformatic approaches. Papers on other related fields are also welcome, such as stem cell biology, genomics, neuroscience, Evodevo, Ecodevo, and medical science as well as related methodology (new or revised techniques) and bioresources.
Resource papers describe a dataset, such as whole genome sequences and expressed sequence tags (ESTs), with some biological insights, which should be valuable for studying the subjects as mentioned above.
Submission of review papers is also encouraged, especially those providing a new scope based on the authors’ own study, or a summarization of their study series.
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