Quorum sensing in Vibrio controls carbon metabolism to optimize growth in changing environmental conditions.

IF 9.8 1区生物学 Q1 Agricultural and Biological Sciences

PLoS Biology Pub Date : 2024-11-11 eCollection Date: 2024-11-01 DOI:10.1371/journal.pbio.3002891

Chelsea A Simpson, Zach R Celentano, Nicholas W Haas, James B McKinlay, Carey D Nadell, Julia C van Kessel

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引用次数: 0

Abstract

Bacteria sense population density via the cell-cell communication system called quorum sensing (QS). The evolution of QS and its maintenance or loss in mixed bacterial communities is highly relevant to understanding how cell-cell signaling impacts bacterial fitness and competition, particularly under varying environmental conditions such as nutrient availability. We uncovered a phenomenon in which Vibrio cells grown in minimal medium optimize expression of the methionine and tetrahydrofolate (THF) synthesis genes via QS. Strains that are genetically "locked" at high cell density grow slowly in minimal glucose media and suppressor mutants accumulate via inactivating mutations in metF (methylenetetrahydrofolate reductase) and luxR (the master QS transcriptional regulator). In mixed cultures, QS mutant strains initially coexist with wild-type, but as glucose is depleted, wild-type outcompetes the QS mutants. Thus, QS regulation of methionine/THF synthesis is a fitness benefit that links nutrient availability and cell density, preventing accumulation of QS-defective mutants.

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弧菌的法定量感应控制碳代谢，以优化在不断变化的环境条件下的生长。

细菌通过称为法定量感应（QS）的细胞-细胞通信系统来感知种群密度。QS 的进化及其在混合细菌群落中的维持或丧失与了解细胞-细胞信号如何影响细菌的适应性和竞争（尤其是在营养供应等不同环境条件下）密切相关。我们发现了一种现象，即在极少培养基中生长的弧菌细胞通过 QS 优化蛋氨酸和四氢叶酸（THF）合成基因的表达。高细胞密度基因 "锁定 "的菌株在极少葡萄糖培养基中生长缓慢，抑制突变体通过metF（亚甲基四氢叶酸还原酶）和luxR（QS转录主调节因子）的失活突变而积累。在混合培养中，QS突变株最初与野生型共存，但随着葡萄糖的耗竭，野生型会取代QS突变株。因此，QS 对蛋氨酸/THF 合成的调控是一种适应性益处，它将营养物质的可用性与细胞密度联系起来，防止 QS 缺陷突变体的积累。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PLoS Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-BIOLOGY

CiteScore

15.40

自引率

2.00%

发文量

359

审稿时长

3-8 weeks

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