{"title":"Establishment of a Minimally Invasive Rat Model of Pulmonary Embolism Using Autologous Blood Clots.","authors":"Ruihao Li, Zuoguan Chen, Yaming Guo, Changduo Wang, Yongpeng Diao, Jing Yang, Yongjun Li","doi":"10.3791/66776","DOIUrl":null,"url":null,"abstract":"<p><p>Pulmonary embolism (PE) is one of the leading causes of cardiovascular death, resulting in a significant socioeconomic burden. Although current treatments primarily focus on anticoagulation and thrombolysis, there is an urgent need for a better understanding of its pathophysiology and the development of new treatment strategies. Animal models play a crucial role in understanding PE and developing new therapies for the disease, with rodents commonly used due to ethical and cost considerations. However, existing rodent models for PE are limited by a lack of standardized procedures, which hampers reproducibility and cross-study comparisons. This study aims to establish a minimally invasive rat model of PE using autologous blood clots. The model features a minimally invasive blood sampling technique, a standardized thrombus generation procedure, and minimally invasive vein access. Additionally, protocols for quantifying infarcted areas and visualizing the pulmonary arterial tree are provided. These procedures aim to improve the reliability of rodent models for studying PE progression and facilitate the development of novel treatments.</p>","PeriodicalId":48787,"journal":{"name":"Jove-Journal of Visualized Experiments","volume":" 212","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Jove-Journal of Visualized Experiments","FirstCategoryId":"103","ListUrlMain":"https://doi.org/10.3791/66776","RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Pulmonary embolism (PE) is one of the leading causes of cardiovascular death, resulting in a significant socioeconomic burden. Although current treatments primarily focus on anticoagulation and thrombolysis, there is an urgent need for a better understanding of its pathophysiology and the development of new treatment strategies. Animal models play a crucial role in understanding PE and developing new therapies for the disease, with rodents commonly used due to ethical and cost considerations. However, existing rodent models for PE are limited by a lack of standardized procedures, which hampers reproducibility and cross-study comparisons. This study aims to establish a minimally invasive rat model of PE using autologous blood clots. The model features a minimally invasive blood sampling technique, a standardized thrombus generation procedure, and minimally invasive vein access. Additionally, protocols for quantifying infarcted areas and visualizing the pulmonary arterial tree are provided. These procedures aim to improve the reliability of rodent models for studying PE progression and facilitate the development of novel treatments.
肺栓塞(PE)是心血管疾病死亡的主要原因之一,造成了巨大的社会经济负担。虽然目前的治疗方法主要集中在抗凝和溶栓上,但人们迫切需要更好地了解其病理生理学并开发新的治疗策略。动物模型在了解 PE 和开发该疾病的新疗法方面起着至关重要的作用,出于伦理和成本方面的考虑,通常使用啮齿类动物。然而,现有的 PE 啮齿动物模型因缺乏标准化程序而受到限制,这妨碍了重现性和跨研究比较。本研究旨在利用自体血凝块建立 PE 的微创大鼠模型。该模型采用微创血液采样技术、标准化血栓生成程序和微创静脉通路。此外,该模型还提供了量化梗死区域和观察肺动脉树的方案。这些程序旨在提高研究 PE 进展的啮齿动物模型的可靠性,并促进新型治疗方法的开发。
期刊介绍:
JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.